structural biology

The paper generates conformational ensembles of the 4E-BP2:eIF4E complex using smFRET and NMR data, revealing dynamic structural changes and new contact regions.

This study identifies CLN8 as a stereospecific GPG acyltransferase critical for BMP biosynthesis, showing its loss causes Batten disease and that S,S-LPG supplementation can restore BMP and improve neurological symptoms.

This study shows how glucose binding reorganizes the conformational and energetic landscape of PfHT1, a malaria parasite transporter, using computational methods and experimental validation.

This study determines the structure of Kv channels bound to 4AP, showing it binds the closed state and enters via membrane-facing fenestrations, explaining its inhibitory mechanism.

This study compares the dynamic properties of IL-1β and IL-1Ra using NMR relaxation to show that their distinct functional roles in IL-1R signaling arise from differences in conformational entropy at the receptor interface.

This study determines the structure of Plasmodium falciparum MyoB, shows it is a slow motor optimized for force generation during invasion, and identifies its light chain as shared with MyoA.

This paper develops a machine learning framework to predict capsid protein binding sites in ssRNA viruses using local geometric features from RNA structures, achieving high accuracy with and without experimental data.

This study identifies distinct determinants of ACKR1 recognition by bacterial toxins, revealing a two-step mechanism involving sulfotyrosine binding and extracellular vestibule interaction.

This paper identifies two lipid-mediated mechanisms of Tissue Factor self-regulation through structural analysis, revealing conformational changes and their impact on FVII binding.

This study shows that phosphoinositide protonation microstates determine AKT1 membrane binding specificity and how mutations alter this interaction.

The paper determines the cryo-EM structure of the human mitochondrial RNA degradosome, revealing a unique helicase-nuclease coupling mechanism.

This paper investigates how the L405D mutation in SARS-CoV-2 nsp13 decouples ATP hydrolysis from RNA translocation by altering conformational dynamics.

This paper discovers and structurally analyzes a novel -1,2-glucosidase from Arthrobacter humicola A8F5, revealing its unique mechanism and structural features for cleaving rare -1,2-glucosidic bonds.

The paper presents the cryo-EM structure of AsCas12a bound to a pseudo-DNA guide and RNA target, revealing its mechanism of DNA-guided RNA recognition.

This study shows that CgMscS from Corynebacterium glutamicum opens at higher membrane tension than EcMscS and uses a distinct gating mechanism not based on the 'lipids-move-first' model.

This study investigates the N2-fixing system of Methanocaldococcus infernus, revealing structural insights into its thermostable nitrogenase and unifying mechanistic principles across nitrogenase types.

This study characterizes the structure of the non-tailed vibriophage NO16, revealing its capsid organization and potential host interaction mechanisms.

The paper identifies a conserved NADase domain, NIR, in bacterial immunity and eukaryotic proteins like human TEP1, linking bacterial defense mechanisms to eukaryotic functions.

The paper identifies an alternatively-spliced form of CHC17 (CHC17-SAS) that compensates for the absence of CHC22 in some vertebrates, enabling GLUT4 trafficking.

This study identifies the structural basis of BRD4 interaction with P-TEFb and shows how BRD4 and AFF4 regulate P-TEFb activity through distinct mechanisms.

This paper reports a 13-subunit c-ring in the Chlamydomonas chloroplast ATP synthase, which lowers the H+/ATP cost of carbon fixation compared to the 14-subunit structure in spinach.

The paper identifies ligands with varying steric profiles that exhibit different β-arrestin recruitment capacities at GPR84, revealing a mechanism for controlling signaling bias.

This study uses molecular dynamics simulations to show how pH and temperature together destabilize dengue virus envelope dimers, revealing a 'compensatory embrace' mechanism and potential targets for antiviral drugs.

This study determines the structural basis of Trm10's specific tRNA methylation by capturing its post-catalytic states using cryo-EM and MD simulations.

The paper investigates the structural and functional role of QueC-family protein QatC in the QatABCD anti-phage system, showing its role in protein modification similar to type IV CBASS.

This study uses HS-AFM to directly observe conformational changes in FGFR1 during signal transduction, showing that autophosphorylation enables dynamic interactions with FRS2.

This study reports co-crystal structures of a bioluminescent energy-transfer complex in Renilla reniformis, revealing the spatial arrangement of donor-acceptor chromophores and their role in efficient radiationless energy transfer.

This study investigates how metabolic signals regulate glutamine synthetase AMPylation in Herbaspirillum seropedicae and reveals the structural mechanism of enzyme inhibition by AMPylation.

This paper introduces a generic numbering scheme for TMEM16 scramblases to enable comparative analysis across paralogs with different sequence numbering systems.

This study determines the cryo-EM structures of DNMT1 bound to pUG-fold RNA, revealing two RNA-binding modes and their role in inhibiting DNMT1 activity.