Plant tissue culture and regeneration

This study develops a micropropagation protocol for Dendrobium moniliforme and confirms genetic stability using RAPD and ISSR markers.

The paper uses CRISPR/Cas9 to edit the LePPO1 gene in Lithospermum erythrorhizon, showing it is involved in shikonin biosynthesis and explains the role of copper in the process.

The paper optimizes Agrobacterium-mediated transformation in heirloom tomato cultivars to enable CRISPR/Cas9 genome editing for trait improvement.

This study improves perennial ryegrass transformation by using developmental regulator genes, enabling efficient plant regeneration across genotypes.

The paper ranks plant callus metabolomes for health and cosmetic applications by identifying functionally relevant metabolites using a new pipeline.

This paper introduces VAST, a method improving transient gene expression in plant seedlings, showing enhanced efficiency across multiple species.

This study develops an optimized Agrobacterium-mediated wheat transformation protocol using GRF4-GIF1, enabling efficient, genotype-independent transformation across multiple wheat cultivars.

The paper demonstrates stable transgene and YFP expression inheritance in cacao progeny using transgenic plants as pollen or ovule donors.

The paper optimizes three wheat transformation methods using CRISPR/Cas9 to target TaARE1-D, achieving high transformation efficiencies and improving yield traits in wheat mutants.

This paper identifies a strain of Spirodela polyrhiza suitable for genetic transformation and genome editing, provides detailed procedures, and offers tools for genome exploration and CRISPR design.

This paper develops an efficient in vitro regeneration system for sweet orange that improves rooting and transgenic plant recovery.

This study identifies STM as a key regulator for the transition of shoot progenitor cells to productive shoot progenitor cells in Arabidopsis tissue culture.

This study develops a method using developmental regulators to improve soybean transformation and CRISPR-mediated genome editing efficiency.

The paper shows that BAP concentration needed for efficient shoot regeneration in leaf lettuce varies between white and brown seed cultivars.

This study enhances Agrobacterium-mediated genome editing in Arabidopsis by optimizing floral stages and engineering Agrobacterium to evade plant immunity, achieving high transformation efficiency without antibiotic selection.

This study compares phytochemicals and antioxidant activity between callus cultures and leaf extracts of Cinnamomum camphora, finding higher antioxidant enzymes and phenolics in callus cultures.

The paper introduces a floral injection method to improve Agrobacterium-mediated transformation in Mimulus guttatus, enabling transformation of both coastal perennial and inland annual ecotypes.

This study demonstrates that viral vectors can enhance leaf transformation in Sorghum bicolor by delivering morphogenic regulators, achieving higher embryonic calli formation rates than conventional vectors.

The paper develops an efficient transformation method for winter-type rapeseed using WUSCHEL from Beta vulgaris, enabling CRISPR/Cas9 editing of gene families and phenotypic analysis.

The Q-Warg pipeline quantitatively compares protoplast culture conditions to optimize cell wall recovery and viability, offering a user-friendly tool for unbiased analysis.

This study shows that expressing a GRF-GIF chimera in tomato accelerates shoot regeneration and increases transgenic plant recovery, improving genome editing efficiency.

This study uses single-cell mass spectrometry to reveal metabolic heterogeneity in apple callus cells and shows that abiotic stress increases triterpenic acid production.

This study shows ESR2 promotes cytokinin biosynthesis and signaling inhibition, which together drive green callus formation in plants.

The paper investigates using metal-organic frameworks (MOFs) to elicit secondary metabolite accumulation in Aquilaria crassna callus cultures, finding that certain MOFs significantly increase metabolite production.

This paper presents a semi-automated protocol for Agrobacterium-mediated plant transformation in Marchantia polymorpha, improving efficiency and reducing costs for high-throughput experiments.

This paper introduces a new method for transforming Marchantia polymorpha using chopped thalli, achieving high efficiency and scalability for genetic studies.

The paper assesses airflow's impact on plant callus cultures using multiomics data from tobacco, rice, and bamboo species, revealing species-specific and within-species differences.

This paper develops a fed-batch cultivation method for Viola odorata plant cells, achieving higher biomass production and demonstrating antiplasmodial activity comparable to natural extracts.

The paper develops new integrative vectors for Agrobacterium rhizogenes transformation, improving efficiency and enabling functional analysis of soybean GmTML gene family members.

This study develops a CRISPR-based gene editing approach in almond using hairy root transformation, achieving target gene knock-out in ERF74 and GAI.