Fungal and yeast genetics research

This study examines the time-resolved changes in the cell wall proteome of Saccharomyces cerevisiae during batch fermentation, revealing dynamic regulation linked to metabolic transitions and nutrient availability.

The paper examines filamentation morphology evolution in Saccharomycotina yeasts using integrated genomic, metabolic, and environmental data from 1,154 strains, finding that filamentation is common and linked to phylogeny and environmental factors.

This study investigates the uptake mechanisms and physiological effects of furanic compounds in budding yeast, revealing distinct toxicity pathways for different compounds.

The paper identifies a conserved short linear motif that binds to Spa2/GIT1 family proteins, affecting tip growth and localization in yeast.

The paper identifies and classifies GPCRs in 1,357 fungal species, revealing Pth11-like GPCRs as predominant and identifying potential novel fungal GPCRs using a custom CNN and AlphaFold Multimer.

This study measures proteome changes in S. cerevisiae during recombinant laccase expression and finds strain-specific responses linked to protein yield.

The paper improves genotype inference in yeast to reveal that LOH rates vary due to trans and cis factors, challenging previous findings.

This study identifies the TOR signaling pathway and Ino80 chromatin-remodeling complex as key regulators of hypoxic adaptation in Candida albicans by coordinating phosphate homeostasis and chromatin accessibility.

The paper introduces RoCi, a method for integrating multiple directly repeated gene-expression cassettes into a defined genomic locus in fungi, resulting in stable and high-yield production of metabolites and enzymes.

The paper examines non-redundant roles of paralogous proteins in yeast glucose-sensing pathways through gene deletion and multi-omics analyses.

The paper observes decreased molecular crowding during yeast to filamentous growth transition in C. albicans, linked to reduced ribosome concentration and increased cytoplasmic volume.

The paper investigates recombination suppression around the mating-type locus in Schizothecium fungi, finding evolutionary strata associated with a diploid-like life cycle in pseudo-homothallic species.

This study investigates the TOR pathway in Neurospora crassa, showing that VTA and GTR2 regulate TOR activation by amino acids and its localization to vacuolar structures.

This paper introduces a method to regulate proteolysis of GFP fusion proteins in A. fumigatus using a nanobody-Rnf4 fusion protein, enabling direct protein level manipulation.

This study shows that Trr1 depletion in C. albicans increases sensitivity to antifungals and cell wall stress by disrupting metabolic pathways and cell wall synthesis.

The paper investigates the role of HID1 in cell proliferation using Schizosaccharomyces pombe, finding that hid1{Delta} mutants exhibit reduced proliferation due to metabolic stress and compromised Golgi function.

The paper shows that heterochromatin epimutations in fission yeast cause mitochondrial dysfunction and antifungal resistance by repressing genes involved in mitochondrial function.

This study shows lactate enhances iron assimilation in C. albicans by modulating iron transporter gene expression and promoting iron accumulation under iron-limited conditions.

The paper investigates how acidocalcisome-like vacuoles regulate cytosolic phosphate levels through polyphosphate synthesis and feedback mechanisms.

The paper constructs a barcoded insertional mutant library in Trichoderma atroviride to enable high-throughput functional genomics in filamentous fungi.

This paper introduces an ABA-based anchor-away system for reversible nuclear protein depletion in yeast, overcoming limitations of the rapamycin-based system.

This study identifies Tsa1 as a regulator of PKA activity during glucose depletion, showing its role in metabolic adaptation and cell cycle control in yeast.

The paper identifies a C. glabrata protein Yhi1 that facilitates inter-species interaction with C. albicans by inducing hyphal growth, regulated by mating signaling pathways.

PIONEER is a yeast-based platform for screening genetically encoded protein regulators by displaying them in the periplasmic space to assess their function through signaling.

The paper uses time-lapse microscopy to study secretory vesicle dynamics in Botrytis cinerea, revealing two modes of vesicle accumulation at hyphal apices.

This study shows that S. eubayanus adapts to long-term cold starvation through transcriptional reprogramming, not genetic mutations, leading to improved cryotolerance and growth.

This study characterizes ORF19.7608 (PPP1), a biofilm-induced gene in Candida albicans, showing it localizes to punctate structures under biofilm conditions but is not essential for biofilm formation or stress response.

This study investigates the role of a horizontally acquired genomic region in yeast adaptation by analyzing its molecular and phenotypic effects across different genetic backgrounds.

The paper challenges the 'one nucleus, one whole genome' rule by showing that Neurospora crassa distributes haploid chromosomes unevenly across multiple nuclei.

This study investigates how ploidy and genetic background influence stress tolerance in yeast hybrids, finding condition-dependent effects on heterosis.