Pluripotent Stem Cells Research

This study shows that in vivo maturation of MLP-KO iPSC-CMs recapitulates DCM phenotypes, while in vitro maturation does not.

The paper shows that inducing DOT1L in serum/LIF ESCs increases RNAPII accumulation at transcription start sites, mimicking 2i/LIF conditions, but without altering steady-state RNA expression.

This study uses Perturb-seq to analyze how varying levels of pluripotency regulators affect hESC self-renewal and differentiation through single-cell RNA-seq.

This paper develops an in vitro model of the human chorion using blastocysts to generate trophoblast organoids and extraembryonic mesoderm cells, enabling study of placental development and pathophysiology.

The paper describes a new NHGRI Sample Repository containing seven iPSC lines generated from PBMCs, with genomic data for studying genetic variation and its functional implications.

This study shows STAT3 is essential for esophageal progenitor cell development and epithelial morphogenesis in humans, but not in mice.

The paper shows that inhibiting ERK signaling expands epiblast cells capable of becoming naive pluripotent stem cells in humans, while FGF stimulation expands hypoblast markers.

The paper identifies Polycomb-regulated gene expression and chromatin state differences in iPSCs that correlate with differentiation efficiency.

This study investigates the developmental potential of rabbit ICMs to differentiate into extraembryonic lineages, finding that early-stage ICMs can form a blastocyst-like structure with TE and primitive endoderm, while later-stage ICMs lose TE specification ability.

This study shows that cannabidiol can reprogram peripheral blood mononuclear cells into neural stem/progenitor-like cells through direct reprogramming.

This study designs new pluripotency maintenance protocols by leveraging cellular response dynamics to reduce drug exposure while maintaining pluripotency in mouse embryonic stem cells.

This study shows that juxtaposing amnion-like cells with human pluripotent stem cells initiates gastrulation behaviors, including primitive streak formation and mesendodermal differentiation.

The paper programs human neuron subtypes using combinatorial patterning and scRNA-seq to identify diverse neuron types and their developmental signaling networks.

The paper demonstrates a novel protocol to generate motile cardiomyocyte aggregates from mouse embryonic stem cells, enhancing their contractility and motility for biobot development.

This study develops a single-step transposon-based method to generate pure GABAergic neurons from iPSCs, showing they mature synaptically and form functional networks.

This study shows that overexpressing MIF in hypoimmunogenic iPSC-derived NPCs reduces NK cell attack and improves engraftment in SCI models.

The paper develops a 2D/3D hybrid organoid system for high-throughput drug screening in iPSC cardiomyocytes, validated using doxorubicin to assess cardiotoxicity and electrophysiological effects.

The paper shows that mesendodermal cells induced from mouse embryonic stem cells fail to contribute to heart formation when injected into blastocysts.

This study generates and tests astrocyte progenitor cells from hiPSCs, showing their safe survival and integration in spinal cord transplants without adverse effects.

The paper shows that TFAP2A+ progenitor cells generate amnion, germline, and mesoderm through BMP signaling and basement membrane cues in vitro.

This study shows that maturing human cardiac organoids enhances disease modeling and drug discovery by mimicking in vivo development and enabling therapeutic testing for DSP cardiomyopathy.

This study develops a method to generate human liver bud organoids with functional sinusoidal vessels, demonstrating their potential for coagulation disorder therapies.

This paper enhances human pluripotent stem cell (hPSC) proliferation to improve chimaerism in human-mouse chimaeras, resulting in viable pups with integrated hPSC-derived tissues.

This study shows that donor age impairs the vasculogenic potential of hiPSC-derived endothelial progenitors, with older donor cells forming less functional vascular structures and showing epigenetic and transcriptomic differences.

The paper shows that extended NGN2 expression in iPSCs improves neuronal culture purity by reducing heterogeneity caused by suboptimal differentiation.

The paper generates hiPSC-CM from male and female donors and finds sex dimorphism in calcium handling and gene expression without hormone exposure.

The paper develops a multi-omics guided differentiation protocol to generate PD-vulnerable midbrain dopaminergic neurons, showing improved yield and functional recovery in mice.

This study generates non-viral canine induced pluripotent stem cells (iPSCs) from umbilical cord fibroblasts, confirming their pluripotency and differentiation potential.

This study shows that FOXM1 inhibition promotes terminal differentiation of human iPSC-derived hepatocytes by enhancing polyploidization and metabolic maturation through the Wnt/β-catenin pathway.