Pluripotent Stem Cells Research

The paper introduces DCM-time machine (DCM-TM) to track gene and enhancer activity during human iPSC differentiation, showing its ability to label active elements and correlate methylation changes with cell state transitions.

The paper investigates whether p38-MAPK inhibition affects primitive endoderm differentiation in mouse blastocysts through ICM autonomous mechanisms or cavity expansion defects.

This study shows that culturing pluripotent stem cells in microscale droplets promotes differentiation into three germ layers and organoid formation, with enhanced tissue patterning and self-organization.

The paper establishes stable 2C-like cells from ESCs, showing persistent MERVL expression and expanded developmental potential through epigenomic and transcriptomic analysis.

The paper describes a method to generate human embryo-like structures by pre-patterning stem cells and mixing them, resulting in models with extended anterior-posterior organization and neural features.

This paper develops a human iPSC-derived microfluidic platform to model corticospinal tract connectivity and pathology for neurodegenerative disease research.

The paper maps single-cell dynamics of endothelial-to-hematopoietic transition from iPSCs and identifies FGF signaling modulation to improve hematopoietic output.

This study shows that co-culturing human iPSC-derived sensory neurons with rodent satellite glia enhances their differentiation and maturation, leading to more mature phenotypes and improved disease modeling.

The paper identifies Asgard, a STAT3-regulated lncRNA, which controls microRNA biogenesis and sequestration to maintain naive pluripotency in mouse embryonic stem cells.

This paper develops a heart-on-a-chip platform using human iPSC-derived cardiomyocytes and a multi-electrode array to evaluate drug-induced cardiotoxicity with high reproducibility and multi-angle analysis.

The paper shows AKNA regulates neural stem cell fate by modulating chromatin states through interactions with KDM6B and signaling pathways.

This study investigates how metabolic maturation affects molecular and metabolic states in hiPSC-derived cardiomyocytes with TNNT2-linked hypertrophic cardiomyopathy, revealing variant-specific disruptions in metabolic pathways and gene regulation.

The paper presents a brain organoid atlas of 352 patient-derived iPSCs from neurodevelopmental disorders, showing disease-specific cellular defects in organoids.

This paper identifies Hic2 as a gene that enhances iPSC generation by suppressing KLF4-dependent epidermal gene expression during reprogramming.

The paper introduces UNMASC, a mathematical model to analyze non-monotonic cytokine interactions in T cell differentiation, showing synergistic and antagonistic effects in cytokine combinations.

This study generates human pluripotent stem cell-derived AVN-like pacemaker cells that replicate AVN function in vivo, suggesting their potential as biological conduction bridges for treating AV block.

The paper integrates scRNA-seq data to identify metabolically quiescent 8CLCs resembling human 8-cell stage embryos and characterizes their gene expression networks.

SteMClass is a DNA methylation-based classifier that accurately identifies iPSC differentiation states across protocols and datasets.

This paper shows that extending FGF9 treatment in iPSC-derived kidney organoids reduces off-target cartilage without affecting renal structures.

This study develops bidirectional pluripotent stem cells (BPSCs) capable of generating both trophoblast and epiblast lineages, enabling the recapitulation of post-gastrula development in vitro and in vivo.

This study generates DRG-like neurons from hiPSCs using NGN2 overexpression and patterning factors, which respond to type 2 cytokines involved in itch.

This study develops iPSC-derived prostate organoids with and without BRCA2 mutations, showing tumorigenic changes and cancer markers in mutated organoids.

The paper compares WNT/{beta}-catenin signalling and TCF/LEF gene expression in human and mouse pluripotent stem cells, revealing species-specific differences in early development.

This study characterizes a new pluripotent state in RSeT hESCs, showing resistance to naive and formative conversion and distinct transcriptomic and signaling features.

The paper describes the generation and characterization of a stable human iPSC line from peripheral blood to serve as a control resource for disease modeling and regenerative medicine.

Toti is a multi-omics database that provides epigenetic and transcriptomic resources for studying totipotent stem cells, offering tools for exploration and analysis.

This study shows that mechanical stretching improves the maturation and differentiation of human iPS cell-derived cardiomyocytes co-cultured with fibroblasts.

The paper generates and validates CRISPR-Cas9 knockout hiPSC lines for Betacellulin to study its role in the EGF system and schizophrenia.

The paper compares metabolic stress resilience of hESC and hiPSC-derived neurons, finding hESCs show greater recovery capabilities under stress conditions.

This paper identifies ICAM-1 as a novel hypoimmune target in human pluripotent stem cells, showing that its knock-out reduces immune cell adhesion and enhances graft survival.