Pluripotent Stem Cells Research

Functional Mammary Gland Organoids From Human Induced Pluripotent Stem Cells

This study develops a method to generate functional mammary gland organoids from human induced pluripotent stem cells, showing they can form layered epithelium and respond to hormonal signals.

Epigenetic Remodeling Increases Plasticity of Bovine Fibroblasts Without Improving Embryo and Stem Cell Development

This study evaluates a multi-target small-molecule cocktail, GUSTC, to reduce repressive heterochromatin marks in bovine fibroblasts, enhancing reprogramming potential without improving embryo development.

A human induced pluripotent stem cell-based high-throughput screening platform for teratogenicity hazard identification

Four-model hiPSC screen in 384-well imaging reached 83% accuracy (88% sensitivity, 75% specificity) on 130 scored compounds from a 146-compound reference set, scalable to hundreds of compounds monthly.

Generation of human iPSC-derived motor neurons with defined regional identities across the rostrocaudal axis

This study develops three protocols to generate human iPSC-derived motor neurons with defined hindbrain, cervical, and lumbar identities, showing graded rostrocaudal specification and region-specific features.

Complex I is essential for embryonic stem cell proliferation through NADH-mediated redox signaling

This study shows that Complex I is essential for ESC proliferation via NADH-mediated redox signaling, independent of energy or ROS levels.

Transient Partial Inhibition of DNA Polymerase α Promotes Chromatin Plasticity and Enhances Human Cell Reprogramming

Transient low-dose inhibition of DNA polymerase α enhances human cell reprogramming by promoting chromatin plasticity while maintaining genomic stability.

Gradient-Based Regulation of Activin A Directs Multilineage Liver Organoid Development from Human Pluripotent Stem Cells

This paper shows that adjusting Activin A levels in a 3D bioreactor system can generate liver-like structures from human stem cells, mimicking early liver development.

Reconstruction of the human nigrostriatal pathway in vitro reveals target-dependent dopamine neuron maturation

The paper introduces a human stem cell-based model of the nigrostriatal pathway that mimics in vivo connectivity and reveals target-dependent dopamine neuron maturation.

Genetic control couples TGFβ-independent pluripotent stem cell renewal to deterministic forward programming across germ layers

This paper introduces GERALT, a genetic circuit that enables TGFβ-independent hiPSC renewal and inducible lineage programming, improving differentiation efficiency and reducing medium complexity.

Roles of Neuromedin-U and Endothelin-1 in human cardiac organoid differentiation

This study identifies Neuromedin-U (NMU) and Endothelin-1 (ET-1) as key signaling factors influencing cardiomyocyte differentiation and electrophysiology in human cardiac organoids.

KLF17 acts as a dual reporter for tracking naive pluripotency and 8-cell-like states

The paper uses KLF17 expression levels to track transitions between primed, naive, and 8-cell-like states in human pluripotent stem cells.

Generation of hepatocyte-like cells from sheep embryonic stem cells

This study generates hepatocyte-like cells from sheep embryonic stem cells using a serum-free method, showing hepatic functions and gene expression patterns.

Direct reprogramming of human monocytes generates blue light- and leptin-responsive ipRGC-like neurons

This study directly reprograms human monocytes into ipRGC-like neurons and identifies factors that enable this conversion.

Generation of an induced pluripotent stem cell line, LCSBi021-A, from a Parkinson's disease patient harboring the missense mutation c.213C>G (p.Ser71Arg) in the RAB32 gene

The paper generates iPSCs from a Parkinson's patient with a RAB32 mutation and confirms their pluripotency and differentiation potential.

Vascularized Organoids Emerge from a Heart-Foregut-like Intermediate Recapitulating Early Human Development

This study shows that heart/foregut-like structures form from human pluripotent stem cells, developing into vascularized cardiac organoids with multiple cell types.

Epigenomic heterogeneity and spatial organization during human reprogramming

This study examines epigenomic and spatial changes during human reprogramming, finding distinct molecular and spatial patterns in productive versus unsuccessful trajectories.

Establishment of Rabbit Formative-like Pluripotent Cells by Bidirectional Modulation of WNT Signaling Pathway

This paper establishes a culture system called KFiC to generate and maintain formative-like pluripotent rabbit embryonic stem cells with developmental plasticity and cross-species compatibility.

RhythmWriter: GPU-accelerated Contractility Quantification for Large Datasets

RhythmWriter quantifies contractile motion in hiPSC-CMs, enabling high-throughput drug screening and maturation assessment.

The Genetic Discovery (GeDi) resource: Deep characterization of a multi-ancestry panel of human reference iPSCs for functional genomic studies

SP2 impedes the pluripotent-to-totipotent state transition in an RA-dependent manner

This study identifies SP2 as a barrier to the pluripotency-to-totipotency transition in mouse embryonic stem cells, showing its role in promoting alternative developmental programs.

Impacts of different SETD1A mutations and genetic backgrounds on human induced pluripotent stem cell-derived hippocampal neurons

This study examines how different SETD1A mutations and genetic backgrounds affect human hippocampal neurons derived from hiPSCs, revealing divergent molecular and cellular phenotypes and contrasting mutation impacts.

A synthetic xeno-free extracellular matrix surrogate supports robust human induced pluripotent stem cell maintenance and multilineage differentiation

This study demonstrates that BIOINSTRUCT, a synthetic extracellular matrix surrogate, supports robust human iPSC maintenance and multilineage differentiation comparable to Matrigel.

STEER-seq: a recombinase based platform for pooled multimodal single-cell perturbation screening

STEER-seq enables pooled single-cell perturbation screening in hiPSCs by using defined genomic integration and barcode tracking to study transcription factor effects on cell identity.

Multi-omics analyses reveal environmentally adaptive cell-state remodeling during human iPSC biomanufacturing

The paper shows that biomanufacturing conditions alter molecular states in hiPSCs without losing pluripotency, linking these changes to developmental programs.

Glia-enriched human brain organoids for studying developmental alterations in leukodystrophies

This study uses human oligodendrocyte spheroids to model leukodystrophies and identifies disease-specific transcriptional changes and lineage-specific vulnerabilities.

Engineering developmental transitions during human neurulation through modular integration of multimodal spatial cues

This paper introduces MoSAIC, a platform that integrates spatial cues to guide human neurulation in vitro, enabling the study of developmental transitions through controlled mechanical and chemical perturbations.

Simplified In Vitro Generation of Human Gastruloids for Modelling Early Development

This paper optimizes a protocol for generating human gastruloids by adjusting culture conditions, improving efficiency and cost-effectiveness for studying early development.

Disease-associated mutations directionally modulate epigenetic age in iPSC-derived tissues

This study shows disease-associated mutations can directionally alter epigenetic age in iPSC-derived organoids, providing a model to study aging and disease interactions.

Multi-omic analysis reveals maturation programs in human pluripotent stem cell-derived cardiomyocytes during long-term culture

This study identifies molecular pathways and regulatory programs associated with hPSC-CM maturation through multi-omic profiling during long-term culture.

Generation of an induced pluripotent stem cell line from a patient with immune checkpoint inhibitor-induced myocarditis and concurrent type I diabetes

The paper describes the generation of an iPSC line from a patient with immune checkpoint inhibitor-induced myocarditis and type I diabetes, highlighting its pluripotency and potential for studying disease mechanisms.