Pluripotent Stem Cells Research

This study shows that loss of chromosome 18q in human embryonic stem cells impairs neuroectoderm commitment and cardiac progenitor differentiation through SALL3 downregulation.

The paper develops a SOX10 reporter system to improve the differentiation of oligodendrocytes from iPSCs, enhancing reproducibility and maturity.

The paper derives epithelial organoids from human amniotic and tracheal fluids to model congenital malformations and predict disease outcomes.

This paper proposes a model of interlinked bistable switches governing Oct4 and Nanog dynamics in embryonic stem cells, explaining their expression patterns during differentiation.

The paper identifies regulatory network modules in human stem cells, showing epigenetic and genetic factors influence pluripotency state differences.

This study uses patient-derived iPSCs with a GNAO1 variant to show altered neuronal differentiation and function in cortical neurons.

The paper generates gene expression data from iPSC-derived cardiomyocytes in a multiethnic cohort and makes the results publicly available.

The paper identifies T1LN as a glycan epitope specific to human pluripotent stem cells (hPSCs) that is rapidly downregulated during differentiation, enabling its use for immunodepletion of hPSCs from differentiated cell populations.

This study shows PRAMEL7 interacts with CUL2 to destabilize NuRD complex, promoting ground-state pluripotency in ESCs by reducing repressive chromatin regulation.

The paper identifies molecular factors that mark pluripotency potential in rare somatic cells by analyzing fibroblast reprogramming efficiency.

This study reprograms non-limb fibroblasts into cells with limb progenitor properties using Prdm16, Zbtb16, Lin28, and Lin41.

This study shows that let-7 regulates pluripotency gene expression in Muse cells by inhibiting the PI3K-AKT pathway and suppressing proliferation and glycolysis.

The paper uses partial cellular reprogramming to reduce DNA damage in an ERCC1 mouse model of premature aging, showing restoration of the epigenetic clock and DNA repair processes.

The paper shows that Zim3, used as a CRISPRi effector without guide RNAs, upregulates cardiac ion channel genes in iPSC-CMs, leading to a more mature phenotype.

This study shows that organoid-derived mesenchymal stromal cells support the proliferation and functionality of hPSC-derived small intestinal epithelial stem cells in adherent culture.

This study shows that KDM5 inhibition promotes iPSC-CM maturation by enhancing fatty acid oxidation, oxidative phosphorylation, and myofibrillar organization through epigenetic regulation of key genes.

The paper identifies ESCs co-expressing GATA6 and SOX2, showing their cooperative DNA binding in enhancers and role in lineage priming for embryonic and extra-embryonic differentiation.

This study genetically modifies human pluripotent stem cells to evade immune rejection in mice by knocking out HLA genes and adding inhibitory factors, leading to persistent teratomas in immunocompetent models.

This paper introduces a method to generate reproducible cerebral organoids from iPSCs using a pillar plate platform, improving throughput and reducing variability compared to traditional methods.

This study shows that mouse embryonic stem cells (ESCs) can proliferate without essential amino acids by taking up and digesting exogenous protein, mimicking pre-implantation embryos.

This study identifies a drug cocktail that accelerates maturation of hPSC-derived neurons and other cell types through pharmacological intervention.

This study shows that DNA methylation is critical for restricting neural differentiation but not germline fate in ESCs, with TKO ESCs capable of germline specification.

This paper describes a method to differentiate mouse embryonic stem cells into megakaryocytes using synthetic gene circuits and specific feeder cell cultures.

This study generates cultured beef from bovine embryonic stem cells by establishing a pluripotent cell line, developing differentiation protocols, and creating a plant-cell hybrid scaffold.

The paper examines DNA replication timing in iPSCs, finding that some exhibit delayed replication at heterochromatic regions, which persists through differentiation and may affect their quality.

This paper develops a method to generate genetically engineered adipocytes from murine iPSCs using CRISPR-Cas9 and tests their engraftment and functional properties in vivo.

This study shows NuRD complex coordinates gene expression during lineage commitment by reducing transcriptional noise, priming genes for activation, and maintaining stable states.

This study identifies a Sox2 mutation that enhances Sox/Oct cooperativity, improving iPSC reprogramming efficiency and enabling reprogramming of aged cells across species.

This study establishes an HLA-based iPSC banking system for the Saudi population, demonstrating that a small number of iPSC lines can cover a significant portion of the population's HLA haplotypes.