Advanced Fluorescence Microscopy Techniques

This paper introduces dual-view microscopy to measure single-molecule dipole orientations with improved precision and three-dimensional accuracy.

This paper introduces a photoswitchable HaloTag (psHaloTag) that enables reversible fluorescence control in living cells using light.

This paper develops a sulfonamide rhodamine with reduced self-blinking for extended super-resolution imaging of proteins in living cells.

This paper introduces SPI, a super-resolution microscopy technique that enables real-time, high-throughput imaging with minimal post-processing.

The paper describes the creation of StayRose, a red-shifted variant of StayGold using genetic code expansion, which retains photostability and can be monomerised.

The paper develops photochemical sectioning to enable nanoscale volumetric imaging of whole-mount specimens, demonstrating its application in analyzing axon degeneration and myelination in mouse olfactory bulbs.

The paper introduces SiR-XActin, a far-red fluorescent probe for imaging actin dynamics in live cells with minimal cytotoxicity and without genetic modifications.

This study uses third-harmonic generation microscopy to image plant roots and microbes in their natural environment without labels.

This study develops MD-FSS for high-resolution brain imaging in awake mice, revealing differences in microglial states and vascular dynamics compared to anesthetized conditions.

FETCH enables precise fluorescent labeling of membrane proteins in Drosophila with spatiotemporal control through covalent bond formation.

This study introduces a calibration method for FRET biosensors using barcoded cells to normalize signals and enable reliable comparisons across experiments.

This paper develops a neural network to reduce photodamage in three-photon microscopy, enabling faster and safer imaging of muscle regeneration.

This paper introduces a low-cost smartphone-based microscope capable of single-molecule detection and super-resolution imaging without signal amplification.

The paper introduces META-SiM, a transformer-based model for analyzing single-molecule fluorescence microscopy data, and discovers a new intermediate state in pre-mRNA splicing.

This study uses STED microscopy to improve resolution of Candida albicans ultrastructure, showing better performance than confocal microscopy for tracking sub-cellular components over time.

The paper introduces SBIS, a fluorescent probe for 4D imaging of brown algal cells, enabling 3D visualization of cell morphology during embryogenesis in multiple species.

Nano-org provides a curated resource of single-molecule localisation microscopy data for studying protein nanoscale distributions and their relationships across cell types and conditions.

This paper introduces 3D-SpecDIM, a technique that captures both 3D positional and spectral dynamics of biomolecules, demonstrating its application in mitophagy and cellular blebbing.

This paper develops a single-molecule enzyme activity assay for serine hydrolases using activity-based probes and demonstrates its application in detecting active granzyme B as a biomarker of liver damage.

The paper predicts calcium sparks using label-free SHG microscopy and pix2pix, showing agreement with ground truth data on frequency and location.

This paper introduces squeezed light field microscopy (SLIM), a method for high-speed 3D volumetric imaging, and demonstrates its application in tracking blood cell motion and neural activity in vivo.

This paper introduces time-resolved fluorescent proteins (TRFPs) that enable temporal-spectral resolved microscopy for multiplexed imaging and super-resolution in live cells.

This paper introduces FRET-TTB, a method to track transfected biomolecules in living cells, showing it can measure conformational changes and localization with single-molecule resolution.

This study develops two red genetically encoded potassium indicators (RGEPOs) for real-time imaging of intracellular and extracellular potassium dynamics in various biological systems.

This study shows surface tethering improves accuracy in measuring diffusion within 3D protein condensates by reducing motion artifacts while preserving their native properties.

The paper introduces CaMK2rep, a sensitive biosensor for detecting CaMKII activity, validated in HeLa cells and neurons, showing linear response and no cytotoxicity.

The paper demonstrates 2D nonlinear structured illumination microscopy (NSIM) using rsEGFP2 to achieve sub-80 nm resolution for live-cell actin imaging.

The paper develops FLIPR, a system for absolute fluorescence lifetime measurement in mice, revealing spatial and temporal variations in dopamine signaling.

This paper introduces Deep3DSIM, an upright 3D-SIM system with adaptive optics for deep tissue imaging, achieving high-quality imaging up to 130 micrometers in Drosophila brain.

The paper introduces a multispectral imaging approach for live-cell imaging, enabling accurate unmixing of low signal-to-noise ratio data at video rates.