RNA Research and Splicing

This study removes 45 microexons in zebrafish using CRISPR/Cas9 and finds minimal developmental impact on brain morphology and function, with some exceptions in specific microexons.

This paper shows that FRG1 regulates nonsense-mediated mRNA decay by modulating UPF1 levels through its interaction with the spliceosome and exon junction complex.

This study shows that NMD in astrocytes regulates calcium signaling and synapse function, affecting anxiety-like behavior in mice.

This study identifies the differential roles of SRSF1's RNA recognition motifs in protein interactions using SILAC proteomics.

The paper shows stress causes bacterial BR-bodies to switch from mRNA decay to storage by changing their physical state and function.

The paper introduces Sciege, a method to estimate cell type-specific isoform distributions using bulk and single-cell RNA-seq data, revealing age and Alzheimer's disease-related splicing changes.

This study investigates how membrane binding influences 2D versus 3D phase separation of the protein FUS, showing that membrane binding allows 2D condensates to form at lower concentrations than bulk phase separation.

This study uses single-molecule tracking to show that nanodomains within FUS condensates regulate diffusion and aging, influencing fibrilization and disease progression.

This study shows that inhibiting EJC assembly with EJC-i disrupts mRNA localization and neural stem cell ciliogenesis by affecting multiple post-transcriptional processes.

The paper improves sQTL modeling by incorporating alternative splicing representation and introduces MAJIQTL, finding new sQTLs linked to Alzheimer's disease.

This study identifies cytoplasmic roles of HSATIII RNAs in forming RNA granules and producing proteins during thermal stress recovery.

The paper identifies DDX55 as a novel regulator of 3'UTR isoform diversity through integrative analysis of RNA binding protein depletion experiments.

The paper develops an integrative sequencing strategy to catalog NRXN1 isoforms across human brain tissues and hiPSC organoids, revealing cell-type-specific splicing patterns and therapeutic potential of ASO targeting.

The paper identifies six novel regulators of RNA-binding protein phase transitions in C. elegans oocytes through a targeted RNAi screen.

The paper investigates the role of RNA splicing factors in abscission checkpoint maintenance and identifies a unique RNA landscape associated with ACB formation.

This study examines alternative splicing and transcriptome changes in human PBMCs after LPS stimulation, identifying novel pathways and splicing events involved in inflammation.

This study investigates how intron RNA structure affects gene expression using reporter assays and machine learning, finding that base pairing significantly modulates mRNA and protein levels.

This study investigates FUS-ERG fusion oncoprotein-DNA co-condensates, revealing nested asymmetric phase separation and their potential for DNA-based information manipulation.

This study identifies ORB2 as a regulator of maternal mRNAs during the Drosophila MZT, showing its ZZ domain mediates repression through interactions with post-transcriptional regulators.

The paper introduces SPICE, a framework that uses splicing assays and generative design to create cell-specific gene regulation elements for research and therapy.

The paper identifies intron retention quantitative trait loci (irQTLs) in human tissues and shows their association with GWAS traits and eQTLs, revealing shared genetic regulation and potential NMD effects.

The paper shows phosphorylation modulates deadenylation rates via multivalent interactions in RNA adaptors, enabling graded regulation of mRNA decay.

The paper investigates whether 5' regions of human protein-coding genes contain blueprints for alternative splicing by comparing genes with multiple and single transcript isoforms.

This study uses refractive index mapping to detect dynamic protein condensation in cell nuclei, showing it is sensitive to reversible condensation and reveals differences from fluorescence-based methods.

The paper identifies GAPDH as a non-canonical RNA-binding protein in AML that stabilizes transcripts and promotes translation, revealing its role in tumor proliferation.

The paper identifies a kinetic ruler mechanism where Nab2 protein concentration determines mRNA poly(A) tail length through competition between polyadenylation and RNA-binding.

The paper shows DUX4 induces HSATII RNA accumulation, leading to protein aggregation and disruption of RNA processing pathways in muscle cells.

The paper uses long-read RNA-seq and isoLASER to distinguish cis- and trans-directed splicing events in human and mouse data, identifying thousands of cis-regulated events in disease-relevant genes.

This study shows glucocorticoids increase P-body numbers and alter mRNA translation fate based on transcript sequence features, not abundance.

This study identifies the CaMKII-RNMT axis as a mechanism controlling activity-dependent RNA dynamics and neuronal morphogenesis through phosphorylation and degradation of RNMT.