Cellular transport and secretion

The paper identifies a unique PI(4,5)P2-positive compartment formed during entosis and related engulfment processes, distinct from phagosomes and macropinosomes.

The paper investigates distinct trafficking routes for polarized and non-polarized membrane proteins in Aspergillus nidulans, showing they use different secretory pathways and Golgi dependencies.

This study identifies Rab6a as essential for HPV trafficking from the TGN to the nucleus by facilitating its association with dynein and BICD2.

This study identifies homology in lysosome-related organelle sorting machinery across eukaryotes, showing its origin predates the last eukaryotic common ancestor.

This study identifies rabphilin-3A as a negative regulator of DCV exocytosis through its interaction with SNAP25, showing increased DCV release in its absence.

This paper develops a physical model of the endoplasmic reticulum as an active liquid network to explain its dynamic structure and function.

The paper identifies filopodia-like structures, called hormonemes, as essential for regulated steroid release in Drosophila, showing their role in synchronizing secretion with physiological needs.

This study uses multiplexed DNA-PAINT imaging to reveal cell-type specific subpopulations of late endosomes/lysosomes with unique protein compositions.

This study identifies MICAL-L1's role in recruiting FCHSD2 to endosomes, which is essential for actin branching, fission, and receptor recycling.

This study identifies two distinct regions in the synaptotagmin-1/SNARE complex interface that mediate different functions in neurotransmitter release and shows that Ca2+ triggers a rearrangement of this interface.

This study identifies REEP1 proteins as forming a novel ER-derived vesicular compartment and shows that mutations affecting their curvature-inducing activity lead to relocalization to the bulk ER.

The paper identifies two functionally distinct ARF1 compartments involved in post-Golgi sorting and endocytic recycling, challenging the classical vesicle shuttling model.

This study identifies membrane tension as a key regulator of exo- and endocytic events during plasma membrane wound repair, showing its role in coordinating resealing and homeostasis restoration.

This study shows that LIC1 and RILPL1/2 restrict angiogenesis by promoting VEGFR2 degradation, and their disruption increases angiogenesis through enhanced Rab11-mediated recycling.

The paper investigates the morphodynamics of intracellular organelles by modeling active mechano-chemical cycles in trafficking pathways, showing how fission and fusion events generate active stresses and hydrodynamic flows.

This paper introduces an assay using free-floating liposomes to detect membrane curvature sensing and remodelling by proteins, revealing new insights into domain-specific preferences and activities.

This paper introduces pHluorin-conjugated secondary nanobodies for measuring synaptic vesicle exo- and endocytosis without genetic manipulation.

The paper identifies a novel endocytic recycling mechanism called CARP, which involves clathrin-associated carriers undergoing partial membrane fusion and release.

The paper proposes a framework and model for spontaneous synaptic vesicle exocytosis, showing that presynaptic actin constrains recycling pool density to regulate release rate.

The paper uses fluorescence imaging to show that EGF stimulation causes multiple receptors and regulatory proteins to co-localize at clathrin-coated sites on the plasma membrane of human cells.

This study shows PM curvature promotes ER-PM contact formation in cardiomyocytes via junctophilin-EHD interactions.

This study uses molecular dynamics to model the dynamic formation of the pre-fusion protein-lipid complex involving Syt1, SNARE, and Cpx, revealing conformational transitions and the role of Syt1-Cpx interactions.

This study shows ATG5 and membrane atg8ylation regulate retromer function in GLUT1 trafficking, independent of canonical autophagy.

This study identifies a mechanical circuit in End4p that regulates force transmission during clathrin-mediated endocytosis in fission yeast.

This study shows that AP-4 is required for rapid vacuolar sorting of the borate transporter BOR1 in Arabidopsis under high-B conditions.

This study develops a humanized yeast model to study TRAPP complex mutations, showing that human TRAPPC1 variants cause secretion and autophagy defects in yeast, mirroring human fibroblast defects.

The paper investigates the structural arrangement of SNARE proteins in QabcR complexes and shows that structural exchange is tolerated but some non-cognate exchanges lead to imbalances.

This study identifies the endosomal-multivesicular body (MVB) pathway as the secretion route for Arc capsids, mediated by phosphatidylinositol-3-phosphate (PI3P).

The paper investigates how the actin-spectrin scaffold in axons regulates clathrin-mediated endocytosis, finding that spectrin clearings restrict pit formation and endocytic activity.