Ion Channels and Receptors

Identification of a transient receptor potential channel that is regulated by phospholipid asymmetry

The paper identifies TRPM8 as an ion channel regulated by phospholipid asymmetry and shows that cytoplasmic phosphatidylserine is required for its function.

Mechanisms determining Schistosoma mansoni CRAC channel activation

This study investigates calcium signaling in Schistosoma mansoni by analyzing STIM and ORAI proteins, identifying pharmacological differences that could enable selective targeting of schistosome calcium channels.

Conserved and species-specific STIM autoinhibition revealed by AlphaFold3-guided dissection

The paper uses AlphaFold3 to analyze STIM autoinhibition in human and C. elegans, revealing conserved and species-specific structural features.

Conformational plasticity of human acid-sensing ion channel 1a

This study presents cryo-EM structures of human ASIC1a across different pH levels and conditions, revealing conformational changes linked to channel function.

A novel screening method using CRISPRa and FM 1-43 to identify cation channels

The paper introduces a method combining CRISPRa and FM 1-43 to screen for cation channels by overexpressing targets and detecting activity with fluorescent labeling.

Endosomal MrGPRX1 signaling sensitizes TRPV1 to enhance itch

This study shows MrGPRX1 signals from endosomes to sensitize TRPV1, enhancing itch signaling, and identifies pathways involved in this process.

IP3R-TRPM4 Coupling Determines the Spatial Reach of Pericyte-Mediated Capillary Constriction

The paper shows that IP3R-TRPM4 coupling enables pericytes to control capillary constriction across multiple branches, depending on signal type and strength.

Structural mechanism of Necrocide 1 activation of human TRPM4 that triggers necrosis by sodium overload

This study identifies the molecular mechanism of Necrocide 1 activation of human TRPM4 and explains its species-specific selectivity using cryo-EM, electrophysiology, and cell death assays.

Automated detection of blink reflexes evoked by optogenetic stimulation of TRPV1-expressing corneal nociceptors in transgenic mice

The paper introduces an automated method to detect blink reflexes in transgenic mice using optogenetic stimulation of TRPV1-expressing corneal nociceptors, achieving high accuracy with machine learning classifiers.

The fungicide mancozeb induces astrocyte atrophy and disrupts Calcium signaling via inhibition of Orai1/STIM1-mediated SOCE

This study shows that mancozeb causes astrocyte atrophy and disrupts calcium signaling by inhibiting Orai1/STIM1-mediated SOCE, leading to altered neurotransmission and behavioral changes at concentrations below current safety limits.

Diversity and Spatial Segregation of TRP Channels in Choanoflagellates Provide Insight into the Evolutionary Origin of Animal Sensory Systems

The paper investigates TRP channel diversity in choanoflagellates, finding ancestral TRP repertoires and spatial segregation, suggesting evolutionary precursors to animal sensory systems.

An ancient receptor family illuminates the evolution of animal sensation

This study identifies an ancient sensory receptor family in choanoflagellates that detects bacterial prey, revealing ancestral features of animal sensation before multicellularity.

Cigarette Smoke Extract (CSE) reduces expression of functional TRPV4 channels in primary human bronchial epithelial cells differentiated at an Air Liquid Interface (ALI) in vitro

This study shows that cigarette smoke extract reduces TRPV4 protein expression and function in human bronchial epithelial cells, potentially contributing to COPD.

TRPM4 Couples Mechanical Force to Myogenic Constriction Throughout the Resistance Vasculature

This study demonstrates that TRPM4 channels are essential for mechanotransduction in resistance arteries, mediating pressure-induced smooth muscle cell depolarization and myogenic constriction across cerebral, mesenteric, and skeletal muscle vessels.

Temperature-dependent ligand relocation reveals plasticity of TRPM4 inhibition

This study shows that the inhibitor PBA binds to TRPM4 in different locations at 8°C and 37°C, revealing temperature-dependent changes in ligand recognition.

Piezo2 tension sensitivity and its modulation by alternative splicing

This study investigates how alternative splicing of Piezo2 affects its sensitivity to membrane tension using electrophysiology and microscopy.

An INF2-dependent actin-mediated step in Inositol 1,4,5-trisphosphate receptor cluster formation and activity

This paper identifies INF2-mediated actin filaments as a regulator of IP3R cluster formation and activity, influencing ER calcium release and ER-mitochondrial contacts.

Temperature and intrinsic Ca2+ reshape TRPM4 pharmacology

The paper shows that temperature and Ca2+ alter TRPM4's pharmacology, revealing how these factors influence ligand binding and channel function.

PIGBOS-CLCC1 Interaction Shapes Cellular Calcium Dynamics and Energy Metabolism

This paper identifies PIGBOS as a mitochondrial microprotein that regulates ER-mitochondrial Ca2+ signaling and links Ca2+ dynamics to mitochondrial energy metabolism and stress responses.

Direct analytical estimation of thermodynamic parameters of thermo-TRP channels

The paper develops a method to analytically estimate thermodynamic parameters of thermoTRP channels using algebraic calculations based on patch-clamp data.

Piezo3 is a novel mechanosensitive Piezo ion channel in vertebrates

The paper identifies Piezo3 as a new PIEZO paralog in vertebrates, shows its mechanosensitive function in HEK293T cells, and demonstrates its role in zebrafish mechanosensation and light response.

Characterisation of cold-selective lamina I spinal projection neurons

The paper identifies cold-selective lamina I spinal projection neurons and their connections to brain regions involved in thermosensation.

Optical single-channel recording of CRAC channels with HaloTag and a Ca2+-sensitive ligand

This paper describes an optical method to study CRAC channel gating using HaloTag and a Ca2+-sensitive dye, overcoming limitations of traditional electrophysiology.

Activation by statins unveils two putative agonist binding sites in the pore domain of TRPA1

This study identifies two distinct agonist binding sites in TRPA1 activated by statins and shows competitive interactions between agonists and antagonists.

Functional Evaluation of TRPC6 Missense Variants in Cancer Patients via Molecular Docking Analysis Compared with Patch Clamp Electrophysiology

This study evaluates TRPC6 missense variants in cancer patients using molecular docking and patch clamp electrophysiology to predict functional impact and guide clinical decisions.

CaRPOOL: A Pooled Calcium-Recording CRISPR Screening Platform Identifies CCR7 as a Modulator of Cellular Osmomechanosensing

CaRPOOL combines CRISPRi and CaMPARI2 to identify CCR7 as a modulator of osmomechanosensing through a PIEZO1-dependent pathway.

TRPA1 channel activation by synthetic lipid nanoparticles

This study shows that lipid nanoparticles can activate TRPA1 through multiple calcium influx mechanisms, suggesting a new non-canonical activation pathway.

Deep mutational scan of the pore of the cold-sensing TRPM8 channel

The paper uses deep mutational scanning to study the TRPM8 channel's pore region, revealing how structural elements coordinate gating and ion selectivity in response to menthol and cold.

Harnessing NCX-IP3R-dependent Calcium Oscillations to Regulate Angiogenic Signaling in Endothelial Cells

This paper demonstrates that sodium-calcium cross-talk regulates calcium oscillations in endothelial cells, which control angiogenic signaling and can be manipulated to generate angiogenic responses in engineered tissues.

MDFIC2 is a sensory neuron-specific PIEZO channel auxiliary subunit

The paper identifies MDFIC2 as a PIEZO channel auxiliary subunit specifically expressed in mechanosensitive neurons and shows it modulates PIEZO1/2 mechanosensitivity and inactivation kinetics.