Ion Channels and Receptors

Molecular and Functional Analysis of Calcium Binding by a Cancer-linked Calreticulin Mutant

The paper analyzes calcium binding properties of a cancer-linked calreticulin mutant and shows it retains low-affinity calcium binding and maintains ER calcium homeostasis.

Structural Mechanism of TRPC3 Channel Activation by the Moonwalker Mutation

The paper presents structures of hTRPC3 in resting and open states, revealing how the moonwalker mutation activates the channel by disrupting a polar interaction and inducing structural changes.

Syntaxin11 Deficiency Inhibits CRAC Channel Priming to Suppress Cytotoxicity and Gene Expression in T Lymphocytes Independent of Membrane Trafficking.

This study shows Syntaxin11 primes Orai1 for CRAC channel function, affecting T cell cytotoxicity and gene expression without impacting membrane trafficking.

PKD2L1 channels segregated to the apical compartment are the dual-mode pH sensor in cerebrospinal fluid-contacting neurons

This study shows PKD2L1 channels in the apical process of CSFcNs are sensitive to pH changes, modulating neuronal excitability through both phasic and sustained currents.

TRPM2 is a direct pain transducer

This study identifies neuronal TRPM2 as a direct pain transducer that mediates acute and chronic pain independently of immune TRPM2 and inflammation.

A novel role for TRPV1 in macrophage giant cell formation

This study identifies TRPV1 as a key regulator of macrophage fusion and foreign body giant cell formation, independent of TRPV4.

TRPV4 Promotes Histamine Receptor Signaling in Lymphatic Endothelial Cells

This study shows TRPV4 mediates histamine receptor signaling in lymphatic endothelial cells, enhancing Ca2+ signaling and NFATc1 translocation while cytokine release occurs through distinct mechanisms.

Structural rearrangements underlying the activation of STIM1 by ER calcium depletion

This study investigates how ER calcium depletion triggers structural changes in STIM1 that enable CAD release and Orai1 channel activation through single-molecule FRET experiments.

DHHC21 is a STIM1 protein S-acyltransferase that modulates immune function in vivo

This paper identifies DHHC21 as a STIM1 S-acyltransferase essential for SOCE and immune function, showing its role in ALPS and related diseases.

Conformational plasticity of human acid-sensing ion channel 1a

This study presents cryo-EM structures of human ASIC1a across different pH levels and conditions, revealing conformational changes linked to channel function.

Endosomal MrGPRX1 signaling sensitizes TRPV1 to enhance itch

This study shows MrGPRX1 signals from endosomes to sensitize TRPV1, enhancing itch signaling, and identifies pathways involved in this process.

Structural mechanism of Necrocide 1 activation of human TRPM4 that triggers necrosis by sodium overload

This study identifies the molecular mechanism of Necrocide 1 activation of human TRPM4 and explains its species-specific selectivity using cryo-EM, electrophysiology, and cell death assays.

Cigarette Smoke Extract (CSE) reduces expression of functional TRPV4 channels in primary human bronchial epithelial cells differentiated at an Air Liquid Interface (ALI) in vitro

This study shows that cigarette smoke extract reduces TRPV4 protein expression and function in human bronchial epithelial cells, potentially contributing to COPD.

Piezo2 tension sensitivity and its modulation by alternative splicing

This study investigates how alternative splicing of Piezo2 affects its sensitivity to membrane tension using electrophysiology and microscopy.

An INF2-dependent actin-mediated step in Inositol 1,4,5-trisphosphate receptor cluster formation and activity

This paper identifies INF2-mediated actin filaments as a regulator of IP3R cluster formation and activity, influencing ER calcium release and ER-mitochondrial contacts.

Temperature and intrinsic Ca2+ reshape TRPM4 pharmacology

The paper shows that temperature and Ca2+ alter TRPM4's pharmacology, revealing how these factors influence ligand binding and channel function.

PIGBOS-CLCC1 Interaction Shapes Cellular Calcium Dynamics and Energy Metabolism

This paper identifies PIGBOS as a mitochondrial microprotein that regulates ER-mitochondrial Ca2+ signaling and links Ca2+ dynamics to mitochondrial energy metabolism and stress responses.

Characterisation of cold-selective lamina I spinal projection neurons

The paper identifies cold-selective lamina I spinal projection neurons and their connections to brain regions involved in thermosensation.

Functional Evaluation of TRPC6 Missense Variants in Cancer Patients via Molecular Docking Analysis Compared with Patch Clamp Electrophysiology

This study evaluates TRPC6 missense variants in cancer patients using molecular docking and patch clamp electrophysiology to predict functional impact and guide clinical decisions.

CaRPOOL: A Pooled Calcium-Recording CRISPR Screening Platform Identifies CCR7 as a Modulator of Cellular Osmomechanosensing

CaRPOOL combines CRISPRi and CaMPARI2 to identify CCR7 as a modulator of osmomechanosensing through a PIEZO1-dependent pathway.

MDFIC2 is a sensory neuron-specific PIEZO channel auxiliary subunit

The paper identifies MDFIC2 as a PIEZO channel auxiliary subunit specifically expressed in mechanosensitive neurons and shows it modulates PIEZO1/2 mechanosensitivity and inactivation kinetics.

Stereoselectivity and functional plasticity of a common ligand-binding pocket in TRPM3

This study identifies stereoselectivity and functional plasticity in the TRPM3 ligand-binding pocket using cryo-EM and functional analyses, revealing how ligand structure affects channel gating.

Cnpy1 is a candidate endoplasmic reticulum chaperone of Vomeronasal type 2 GPCRs.

This study identifies Cnpy1 as an ER chaperone essential for V2R GPCR function and Go neuron survival in mice.

STIM1 and Endoplasmic Reticulum-Plasma Membrane Contact Sites Oscillate Independently of Calcium-Induced Calcium Release

The paper investigates STIM1 oscillations in mast cells and finds they occur independently of calcium oscillations and are regulated by ER-PM contact site dynamics.

Cromolyn inhibits PGE2-mediated sensitisation of TRPV1 in a GPR35-dependent manner in sensory neurons

The paper investigates how cromolyn, a GPR35 agonist, inhibits PGE2-induced TRPV1 sensitization in sensory neurons, supporting GPR35 as an anti-nociceptive target.

Ether phospholipids modulate somatosensory responses by tuning multiple receptor functions in Drosophila

This study shows that ether phospholipids modulate mechanosensory and thermosensory responses in Drosophila by affecting PIEZO and TRPA1 channels.

Single-cell transcriptomics reveals targeted modulation of inflammatory repertoire by SOCE blockers

This study uses single-cell RNA sequencing to show that SOCE blockers BTP2 and CM4620 reduce cytotoxicity in CD8 T cells and NK cells while preserving anti-inflammatory programs in CD4 Tregs.

Distances and charges along the Orai1 nexus-TM3 interface control STIM1-binding and pore opening

The paper investigates how distances and charges at the Orai1 nexus-TM3 interface influence STIM1-binding and pore opening in the CRAC channel.

A TRPV4-dependent calcium signaling axis governs lamellipodial actin architecture to promote cell migration

The paper identifies a TRPV4-dependent Ca2+ signaling pathway that regulates lamellipodia actin architecture and promotes cell migration through RhoA and formin-mediated actin assembly.

Ideal efficacy photoswitches for TRPC4/5 channels harness high potency for spatiotemporally-resolved control of TRPC function in live tissues

This paper develops and tests ideal efficacy photoswitch ligands for TRPC4/5 channels, enabling precise spatiotemporally-controlled modulation in live tissues.