Chemokine receptors and signaling

This study identifies functional CXCL17 orthologs in reptiles and shows their loss in birds, using bioinformatics and recombinant protein assays.

The paper models how B cells switch between being attracted and repelled by CCL19 through receptor internalization, showing that changing concentration or inhibiting internalization alters cell direction.

This study shows that ACKR2 regulates eosinophil levels in bone marrow, affecting monocyte differentiation and anti-bacterial immunity.

This study shows that MDA-MB-231 cell morphology affects their chemotactic response to CXCL12 gradients in 3D collagen matrices.

This study identifies CXCL13-CXCR5 signaling as critical for CD8 T cell recruitment and immune organization in ccRCC, with potential as a biomarker and therapeutic target.

This study identifies Ephrin B1 as a key mediator of CXCR4 tumorigenic signaling by promoting its intracellular localization and inhibiting surface expression and signaling.

This study identifies a functional CXCL17-GPR25 system in the coelacanth, supporting its evolutionary origin in ancient fishes and transmission to vertebrates.

This study identifies and functionally characterizes CXCL17 orthologs in amphibians, revealing conserved mechanisms of GPR25 receptor activation.

The paper shows that CXCL1 acts as a G protein-biased partial agonist at CXCR1 but a balanced full agonist at CXCR2, revealing receptor bias in chemokine signaling.

This study identifies and functionally characterizes two CXCL17 paralogs in zebrafish, showing they bind and activate GPR25 and induce chemotaxis.

This study uses inverse agonistic nanobodies to reveal constitutive activity of ACKR3, showing it drives non-canonical signaling and basal cell motility, suggesting therapeutic potential.

This study confirms that CXCL17 activates MRGPRX2, MRGPRX1, and MAS1 independently of its C-terminal fragment, unlike its effect on GPR25.

This study characterizes VUF16840 as a selective inverse agonist of ACKR3 that inhibits constitutive and chemokine-induced signaling by stabilizing the inactive conformation of the receptor.

This study identifies CXCL5 as a factor that increases alveolar-epithelial barrier permeability in acute lung injury, independent of neutrophil recruitment.

This study identifies N-terminal determinants of CCL27 and CCL28 receptor activation and selectivity by analyzing mutations and using AlphaFold models.

This study shows human CXCL17 activates and binds to fish GPR25 orthologs, suggesting conservation of the CXCL17-GPR25 interaction across vertebrates.

This study identifies a non-canonical activation mechanism of CCR9 by CCL25 through structural modeling and mutational analysis, revealing the 30s loop as the key activation region.

This study shows that CXCL12-LD, a dimeric variant of the chemokine, reduces chemotaxis in AML cells by altering receptor signaling and internalization, suggesting potential for improved CXCR4 targeting.

This study identifies C/EBPβ as a key driver of colitis-associated tumorigenesis by promoting neutrophil infiltration via the CXCL1/CXCL2/CXCL5-CXCR2 axis, suggesting it as a therapeutic target.

This study shows that disrupting CXCR4 oligomers in lymphoid cancers reduces migration, survival signaling, and tumor growth, while sensitizing cells to venetoclax.

This study shows that CCR1/2/3/5 deletion reduces CD4 T cell responses but not CD8 T cell responses to influenza virus infection.

This study investigates the dual role of the CXCL10-CXCR3 axis in osteosarcoma, showing it promotes tumor growth but also recruits immune cells, suggesting CXCR3 inhibition could target metastasis.

This study shows GPR15LG binds CXCR4, enhancing CXCL12-induced signaling and promoting cell migration in immune and cancer cells.

This study identifies GPR25 as the receptor for CXCL17 using AlphaFold 3 prediction and NanoBiT-based assays, showing specific activation and structural binding.

This study investigates ligand promiscuity and signaling bias in chemokine receptors by analyzing binding modes and determining cryo-EM structures of receptor-ligand complexes.

This study develops a fluorescent ligand to identify improved antagonists targeting the intracellular allosteric site of CCR7, showing their effectiveness in both recombinant and endogenous settings.

This paper develops an ex vivo model of breast cancer cell invasion in live lymph node tissue, revealing preferential invasion patterns and immune-related resistance mechanisms.

The paper computationally optimizes a peptide inhibitor of the CXCL12/HMGB1 heterocomplex, resulting in a variant with significantly improved affinity and activity.

This study investigates how neutrophil-like HL-60 cells respond to traveling waves of chemoattractants, finding that cell movement depends on wave speed and involves different signaling pathways at the front and back of the wave.

The paper performs a meta-analysis of scRNA-seq data to identify disease-specific and shared chemokine-based cell communication patterns in immune diseases.