Zebrafish Biomedical Research Applications

This paper introduces 2D- and 3D-printed striped drums for measuring optokinetic response, contrast sensitivity, and visual acuity in larval zebrafish, showing equivalent responses to standard methods.

This study shows that Decynium-22 increases anxiety-like behavior in zebrafish by affecting monoamine levels and the uptake2 system, suggesting a role for this system in defensive behavior.

This study quantifies how absent blood flow affects vascular development in zebrafish cerebral and trunk vasculature, finding more severe effects in the brain.

The paper shows that Pdgfrb signaling is essential for mural cell development in zebrafish, similar to mice, and identifies context-dependent roles in vascular structures.

This study identifies Fmn2's role in zebrafish neural circuit development, specifically in the formation of spiral fiber neurons critical for the acoustic startle response.

This study uses zebrafish to investigate glial-vascular interactions during CNS development, showing conserved lineage-specific roles in vessel maintenance and formation.

The paper demonstrates that the lac operator-repressor system functions in zebrafish cells to modulate gene expression.

The paper develops reverse genetic systems to identify essential maternal-effect genes and tests them by disrupting kif5Ba and bucky ball.

This paper demonstrates the use of CRISPR-Cas9 dgRNPs to generate pigment-free, immobile zebrafish embryos and larvae across genetic backgrounds, enhancing live imaging capabilities.

This study identifies eight genes, including Dolk and Kv1.1, that regulate zebrafish startle behavior through a common genetic pathway, with Dolk affecting Kv1.1 protein localization.

This paper introduces a minimally invasive brain injection technique for viral-mediated transgenesis in sticklebacks to study genetic effects on behavior.

The paper uses synthetic CRISPR gRNAs and in vivo pre-screening to identify pro-regenerative genes in spinal cord injury, finding that tgfb1a promotes regeneration by controlling inflammation.

This study shows larval zebrafish use a homeostatic algorithm to adjust their phototactic behavior based on luminance history and environmental changes.

BonZeb provides modular tools for high-resolution zebrafish tracking with support for various experimental configurations and analysis workflows.

This paper describes a new mechanism for forming large diameter blood vessels through endothelial struts that act as scaffolds during vasculogenesis.

This study develops an efficient CRISPR/Cas9 pipeline for identifying genes involved in spinal cord repair in adult zebrafish through mutagenesis and behavioral screening.

This paper develops a CRISPR/Cas9 system for neutrophil-specific gene disruption in zebrafish and demonstrates its utility in studying gene function.

This study examines the behavioral effects of developmental JWH-018 exposure in zebrafish and finds no moderation by disc1 mutations in anxiety-like behaviors.

The paper improves the TAEL optogenetic system for zebrafish, enhancing gene expression induction while maintaining safety, and creates stable transgenic lines for broader use.

This paper generates zebrafish Cre drivers by integrating a 2A-Cre transgene into proneural genes, resulting in functional Cre expression in specific cell populations.

The paper describes a low-cost, modular system for high-throughput zebrafish behavior phenotyping, validated with mutant analysis and best practices for larval growth.

This paper introduces a CRISPR-Cas9 method for rapid generation of F0 biallelic knockouts in zebrafish, enabling quick screening of complex phenotypes.

This study shows that Stag1 and Stag2 differentially affect haematopoietic mesoderm development in zebrafish embryos, with stag1a and stag2b mutants showing distinct hematopoietic defects.

This study identifies mutations affecting spine development in zebrafish, revealing new candidate genes for human spine disorders like scoliosis.

The paper shows that Kit ligands cooperate with erythropoietin to promote erythroid cell expansion in zebrafish and reports ex vivo suspension cultures of zebrafish hematopoietic progenitor cells.

The paper develops a high-throughput phenotyping protocol for zebrafish larvae to assess autism-related mutant models, identifying morphological and behavioral defects in syngap1b and slc7a5 mutants.

This paper uses a zebrafish model to study the in vivo effects of human A20 variants, showing that specific mutations impact A20 function and disease relevance.

This study uses light-sheet imaging to map the origin of vascular endothelial cells in zebrafish gastrulae, revealing contributions from different regions along developmental axes.

The paper identifies perivascular fibroblasts in zebrafish as a novel cell type contributing to vascular stabilization through ECM secretion and pericyte progenitor function.