Photoreceptor and optogenetics research

The paper identifies novel fungal modular rhodopsins through in-silico analysis and suggests their potential roles in light-driven signaling and biosynthetic regulation.

This study uses random mutagenesis to generate diverse aureochrome variants with altered photocycle kinetics and DNA-binding affinity, identifying key residues and creating a repertoire for optogenetic applications.

The paper introduces a method combining robotic electrophysiology and single-cell sequencing to link opsin sequences to their functional properties, demonstrating its efficiency over manual methods.

The paper maps the photodynamic diversity of LOV domains, revealing kinetic diversity and functional classes, and demonstrates computational design of new LOV photosensors.

The paper identifies 984 PYP homologs and uses bioinformatics to find candidate proteins involved in PYP signal transduction, including histidine kinase and GGDEF/EAL proteins.

This paper uses cryo-EM to study dark reversion in PaBphP, revealing asymmetric reversion between protomers and structural mechanisms controlling the reaction.

The paper identifies widespread xanthorhodopsin genes in cyanobacteria and characterizes a unique variant from an Antarctic cyanobacterium that binds hydroxylated lutein, enhancing light-harvesting efficiency in extreme environments.

The paper identifies a conserved carotenoid-binding site in heliorhodopsins, revealing their role in light harvesting through exogenous xanthophyll recruitment due to inverted membrane topology.

The paper shows bacterial opsins function as phospholipid scramblases, independent of retinal, and highlights shared functions across domains of life.

The paper identifies and characterizes far-red-sensitive opsins in mantis shrimp eyes, revealing their spectral tuning mechanisms and photoreversibility.

This paper introduces ROCE to study cell signaling and motility by using light to control and observe cellular responses in various cell types.

The paper identifies 13 novel algal LOV domain proteins with potential applications in opto-biotechnology for controlling translational, ribogenetic, and proteolytic processes.

The paper develops a single-component optogenetic system using DmCRY to control gene expression and generate human portrait images from cultured cells.

The paper identifies a KCR1 variant, C29D, that maintains stable potassium selectivity and provides reliable optogenetic inhibition across species and conditions.

The paper reports cryo-EM structures of two methanogenic Schizorhodopsins, revealing distinct proton transport mechanisms and structural adaptations for thermal stability.

The paper identifies a new family of rhodopsin channels in apusomonads that absorb ultraviolet to blue light and function as anion-selective channels involved in photoavoidance.

This study shows that microbial rhodopsins can transport organic anions, expanding their known substrate range and suggesting new optogenetic applications.

The paper identifies blue-shifted channelrhodopsins from ancyromonads that enable multiplex optogenetics by conducting chloride or cation currents with improved spectral properties.

This study presents cryo-EM structures of ChR024, revealing its color-tuning mechanism and transition from a proton pump to a light-gated channel.

The paper uses a machine-learning model to predict red-shifted channelrhodopsin variants, identifying ChR024 with a maximum absorption wavelength of ~578 nm for optogenetics.

The paper identifies WAChR, an excitatory opsin variant with high sensitivity to indoor light, through high-throughput electrophysiological screening of over 1,750 opsins.

This study shows that expressing short-wavelength cone opsin in RGCs causes hyperpolarization, opposite to mid-wavelength opsin's depolarization, due to different G-protein activation.

The paper shows carotenoids enhance rhodopsin light utilization by accelerating photocycles and light-harvesting in marine bacteria.

This study identifies Lys91 as the key residue determining UV sensitivity in vertebrate Opn5, showing its role in spectral tuning and retinal isomer binding.

The paper uses simulations to show dendrites sharpen optogenetic responses in cortex, improving spatial fidelity of stimulation.

The paper identifies leaky Chrimson expression as a source of false-positive signals in optogenetic functional connectivity experiments and proposes a control to mitigate this issue.

This study develops a data-efficient and interpretable model of optogenetic inhibition using potassium and chloride opsins to analyze how parameters and physiological conditions affect seizure suppression in CA1 neurons.

This paper introduces a computational method to reduce off-target stimulation in two-photon holographic optogenetics by optimizing stimulation sites using adaptive non-negative basis function regression.

The paper presents cryo-EM structures of FArhodopsin and proteorhodopsin, showing no retinal binding despite conserved lysine and similar fold to retinal-binding rhodopsins, with mutations identified that prevent retinal binding.

This paper develops NIR imaging and optogenetic tools using Blvra-/- knockout to enhance light-controlled transcription and in vivo glucose regulation.