Photoreceptor and optogenetics research

The paper develops a multichromatic optogenetic toolbox for light-controlled gene expression in Pseudomonas putida, demonstrating spatial and temporal regulation of pyoverdine biosynthesis.

The paper tests optoGPCRs in Drosophila, finding MosOPN3 provides effective, low-toxicity inhibition with brief light pulses.

This paper introduces a non-canonical amino acid that enables chemical control and spectroscopic readout of photoreceptor dynamics, revealing complex photocycle processes in a blue-light receptor.

This paper identifies a coral opsin, AtAntho2c, that can autonomously regenerate its inactive state through thermal isomerization of retinal, enabling repeated light responses without exogenous retinal.

This study investigates the trafficking and turnover of Chlamyopsin6 in Chlamydomonas reinhardtii, showing its dependence on IFT88 and IFT52 for stability and flagellar targeting.

This study evaluates the use of fast-closing Chrimson for optogenetic cochlear stimulation, showing improved energy efficiency and temporal fidelity compared to other ChR variants.

The paper uses reflection spectroscopy to measure photorelaxations in living butterflies, estimating visual pigment absorption spectra and their isosbestic wavelengths.

This paper introduces HaloNeu, a NIR-II-sensitive calcium channel for deep-brain stimulation, showing effective neuromodulation and symptom alleviation in mouse models.

This paper characterizes the kinetic properties of the optogenetic LiCre-loxP system using in vitro and in vivo experiments, revealing temperature-dependent efficiency and a model for recombination synapse formation.

The paper develops a method to ablate specific synaptic connections in neurons using optogenetic tools, demonstrating its application in zebrafish to disrupt functional connectivity and observe behavioral effects.

This paper introduces Opto-MDMi, a dual-lock optogenetic system that activates endogenous p53 with light, while suppressing basal activity in the dark.

This paper introduces a virtual patient framework to predict perceptual outcomes of optogenetic therapies by linking opsin sensitivity and kinetics to temporal contrast sensitivity functions.

This paper enhances an optogenetic tool called LightR by modifying its VVD domains and linker to create variants with improved dynamic range and faster activation kinetics for better control of protein activity.

This paper introduces BLAST, a light-controlled protein secretion system that enables reversible and precise protein release from the endoplasmic reticulum.

The paper develops an optogenetic method to selectively excite and inhibit projection-specific neurons in marmosets, enabling precise modulation of neural circuits.

This study identifies key residues in photoactivated adenylyl cyclase allosteric signaling using simulations, network theory, and machine learning.

The paper develops a gene circuit that uses light and magnetic stimuli to control gene expression in mammalian cells.

The paper studies a BphP from Rhizobium etli to identify variants suitable for optical imaging, including a bright far-red-shifted BphP and a photoswitching BphP for background suppression in vivo.

This paper presents a computational method to calculate retinal isomerization in real time, revealing an asymmetric potential energy landscape and branched photocycle that align with experimental data.

This paper introduces optovolution, a directed evolution method using light and cell cycle oscillations to evolve dynamic protein functionalities, and demonstrates its application in yeast.

This paper revisits time-resolved resonance Raman spectroscopy with continuous-wave excitation, adapting it to modern equipment for studying retinal proteins.

This study compares three bacterial optogenetic Cre recombinase variants at the single-cell level, revealing heterogeneous activity and reporter-dependent performance.

The paper identifies and characterizes two novel bacterial schizorhodopsins from Antarctic sources, demonstrating their inward proton-pumping activity and revealing distinct evolutionary and functional features.

This study shows that two-photon imaging at 920 nm during optogenetic stimulation increases opsin desensitization in C1V1-expressing neurons, affecting both targeted and non-targeted cells.

The paper improves an optogenetic silencer for axonal inhibition, showing enhanced performance and axonal targeting in mouse models.

This paper introduces MTP2, a photoswitch that modulates bacterial membrane potential, revealing context-dependent bioelectrical circuitry in Bacillus subtilis.

This paper introduces a small red-light-responsive system for controlling protein-protein interactions using a cyanobacteriochrome, showing a >25-fold decrease in binding affinity under red light.

This study uses opto-miniRaf to show ERK signaling acutely modulates neuronal activity across multiple scales in cultured neurons and intact brains.

The paper identifies novel fungal modular rhodopsins through in-silico analysis and suggests their potential roles in light-driven signaling and biosynthetic regulation.