Osamu Nureki

This study uses HS-AFM to show yeast TOM complexes predominantly form trimers that dynamically dissociate into dimers and monomers, revealing structural and dynamic features of the TOM complex.

This study determines the cryo-EM structure of SfApuR2, an ultraviolet-sensitive anion-conducting rhodopsin, and identifies structural features that explain its UV sensitivity and gating mechanism.

This study determines the cryo-EM structure of GPR30 with bicarbonate ions, identifies key residues for activation, and provides insights into G-protein coupling and drug development.

This paper introduces a cuffed guide RNA (cgRNA) that enables miRNA activity-dependent genome editing in human and mouse cells.

This study develops a potent B0AT1 inhibitor effective in a PKU mouse model by targeting an allosteric site.

This paper identifies naturally-encoded signaling bias at C5aR1 and C5aR2 receptors through structural and functional analyses, revealing molecular mechanisms underlying biased signaling in GPCRs.

The paper elucidates the mechanism by which YheS releases SecM-arrested ribosomes using cryo-EM and molecular dynamics simulations.

This paper reports cryo-EM structures of the HHV1 helicase-primase complex bound to DNA and inhibitors, revealing how these drugs block viral DNA replication by locking the helicase in an inactive conformation.

This study determines the cryo-EM structures of DARC bound to chemokines CCL7 and CXCL8, revealing unique binding mechanisms and the role of tyrosine sulfation and allelic variation in chemokine engagement.

The paper identifies widespread xanthorhodopsin genes in cyanobacteria and characterizes a unique variant from an Antarctic cyanobacterium that binds hydroxylated lutein, enhancing light-harvesting efficiency in extreme environments.

This study presents the cryo-EM structure of TOK1, revealing its unique domain architecture and lipid-mediated gating mechanism through structural and functional analyses.

The paper shows MT1, a Gi-coupled receptor, can also couple to Gs proteins, activates the Gs/cAMP pathway under long-term melatonin exposure, and presents the first solved structure of a Gi-coupled receptor-Gs complex.

This paper develops engineered FVIII variants for hemophilia A gene therapy, showing improved secretion, coagulation activity, and efficacy at lower vector doses in mice and macaques.

This study identifies ACE2 diversity in horseshoe bats and shows bidirectional evolutionary shaping between ACE2 and sarbecovirus spike proteins.

This study uses base editing to create a universal FIX variant for hemophilia B treatment, increasing FIX activity in cell and mouse models.

The paper reports cryo-EM structures of two methanogenic Schizorhodopsins, revealing distinct proton transport mechanisms and structural adaptations for thermal stability.

This study investigates the ACE2 tropism of sarbecoviruses across 17 mammals using pseudovirus assays, structural analysis, and phylogenetics to identify genetic factors influencing host range.

This paper develops a single AAV vector system for liver-targeted gene knock-in therapy in mouse models of inherited liver diseases, achieving efficient therapeutic gene insertion without germline transmission.

The paper reports the cryo-EM structure of a blue-shifted channelrhodopsin from Klebsormidium nitens and develops mutants with blue-shifted action spectra.

The paper reports light energy transfer from hydroxylated carotenoids to heimdallarchaeial rhodopsins in marine archaea, showing light-harvesting capabilities in archaea similar to bacterial rhodopsins.

This study identifies a convergent molecular mechanism and species-specific pharmacology in complement anaphylatoxin receptors through structural and functional analyses of ligand-receptor interactions.

The paper reports cryo-EM structures of the ETB receptor bound to the lasso peptide RES-701-3, revealing its inverse agonist mechanism through hydrophobic interactions and conformational stabilization.

This study investigates ligand promiscuity and signaling bias in chemokine receptors by analyzing binding modes and determining cryo-EM structures of receptor-ligand complexes.

This study reports six LAT1 structures bound with ligands, revealing substrate recognition and inhibition mechanisms, including the role of JPH203 as an anticancer drug.

The paper identifies a novel A3R-selective ligand and reveals structural insights into A3R activation, enabling structure-guided engineering of m6A-insensitive A3R for drug discovery.

This study investigates how ETB couples to different G proteins, finding that it interacts with Gi and Gq but not Gs, with specific regions influencing these interactions.

This study reports the cryo-EM structure of the QRFP-GPR103-Gq complex, revealing unique binding mechanisms and insights into RF-amide peptide recognition.

The paper identifies two novel ribosome arrest peptides in E. coli and elucidates their unique mechanism of translation arrest.

This study determines the cryo-EM structure of full-length ERGIC-53 complexed with MCFD2, revealing its tetrameric architecture and mechanism of cargo transport.

The paper uses engineered G protein eGt with Nb35 to improve cryo-EM structure determination of GPCR-Gi complexes, achieving better map quality and reduced anisotropy.