Photoreceptor and optogenetics research

The paper introduces LOOMINA, an optogenetic system that deactivates transcriptional activation in response to blue light, offering high adaptability and compatibility with various effector proteins.

This paper develops a red/far-red light-inducible photoswitch system for optogenetic control of gene expression in mice, demonstrating its application in treating metabolic diseases.

This study investigates how domain insertion in a synthetic enzyme fusion enables light-induced allosteric regulation through localized conformational changes and bidirectional domain coupling.

This study shows that overexpressing ChR2-EYFP in skeletal muscle causes contractile dysfunction, while ChR2-only mice show normal function, highlighting the need for alternative optogenetic models.

This paper introduces OptoCre-REDMAP, a red light inducible Cre recombinase system for bacteria, demonstrating its reliability and flexibility in gene expression control.

This paper develops and tests optogenetic tools in yeast to control phosphate-responsive gene expression using light-sensitive fusion proteins.

This paper characterizes HulaCCR1, a novel BCCR from Lake Hula, showing the ETD motif and extended N/C-termini affect spectral tuning, gating, and membrane localization.

This study shows that optogenetic stimulation activates cortical neurons in a morphology-dependent manner, with lateral spread influenced by neuronal layer and stimulation intensity.

This study presents active state structures of a bistable opsin, JSR1, bound to G proteins, revealing differences in activation mechanisms between monostable and bistable opsins.

The paper introduces a photoswitch called DEL-VPR that enables strong, light-induced gene expression in mammalian cells, surpassing constitutive promoters and improving protein production.

This study identifies molecular mechanisms behind spectral shifts in zebrafish cone opsins using molecular modeling and quantum mechanics, highlighting key residues and structural features responsible for different absorption wavelengths.

This study shows that ectopically expressed human rhodopsin does not respond to X-ray stimulation, unlike visible light, and cannot transduce X-ray signals into neural activity.

The paper identifies unintended neuronal activation in the contralateral visual cortex during optogenetic stimulation and proposes ambient light adaptation to mitigate retinal activation.

This study shows that the all-trans 6.11 retinal analogue activates the invertebrate bistable opsin JSR1 and enables complex formation with downstream signaling partners.

The paper analyzes transient TELC and transmembrane potential in laser flashed bacteriorhodopsin purple membranes, finding a peak transient transmembrane potential of 50 mV.

This paper introduces an optogenetic method for perturbing muscles in transgenic mice, enabling simultaneous EMG recording and optical stimulation to study neuromuscular control.

This study evaluates the use of potassium-selective channelrhodopsins (KCRs) for hyperpolarizing C. elegans cells, finding that their effectiveness depends on illumination parameters and can sometimes induce depolarization.

This paper identifies PdCO as a bistable GPCR with high temporal precision for optogenetic control of neural circuits in vivo.

The paper characterizes new rhodopsins in Geodermatophilaceae actinomycetes, including their absorption spectra and ion-pumping activities.

The paper develops a red light-inducible genetic system in Shewanella oneidensis to control extracellular electron transfer.

The paper develops a high-throughput optogenetic platform combined with machine learning to enable multiplexed control of light-sensitive transcription factors in yeast.

This study evaluates factors affecting luminopsin efficacy and identifies strategies to improve their function through molecular evolution.

The paper investigates the abundance and diversity of dinoflagellate proton-pump rhodopsin (DiPPR) genes in Long Island Sound, finding year-round presence, higher abundance in eutrophic areas, and associations with phosphorus levels.

The paper identifies a potential visual opsin in Lissachatina fulica through in silico methods and validates its spectroscopic properties using quantum mechanical calculations.

This study presents cryo-EM structures of human cone visual pigments in complex with G proteins, revealing their active state and mutations affecting spectral tuning.

This study evaluates kalium channelrhodopsins (KCRs) as optogenetic inhibitors in Drosophila, C. elegans, and zebrafish, showing they are effective and have improved properties compared to chloride-conducting ACRs.

This paper develops an optogenetic gene expression system in Lactococcus lactis using split T7 RNA polymerase linked to Vivid regulators, enabling light-controlled protein expression with high induction and sensitivity.

The paper introduces a database of opsin genotypes and λmax phenotypes and demonstrates regression-based ML models can predict λmax and identify functionally critical sites.

The paper introduces an open-source device for high-throughput optogenetic experiments in microwell plates, enabling automated stimulation, spectroscopy, and feedback control in E. coli.

This paper describes an experimental setup for fast 2-photon stimulation of neurons using holographic patterns to achieve sub-millisecond temporal resolution.