A. Chiki

Deep learning-assisted single-molecule detection of protein post-translational modifications with a biological nanopore

This study uses a biological nanopore and deep learning to detect and distinguish single and multiple PTMs on -synuclein peptides at picomolar concentrations.

Investigating crosstalk among PTMs provides novel insight into the structural basis underlying the differential effects of Nt17 PTMs on mutant Httex1 aggregation

This study investigates how PTMs in Nt17 of mutant Httex1 affect aggregation, showing that oxM8 delays aggregation and that combinations with AcK6 inhibit fibrillization, with structural insights from spectroscopy and simulations.

Site-specific phosphorylation of Huntingtin exon 1 recombinant proteins enabled by the discovery of novel kinases

This paper introduces a method to produce site-specifically phosphorylated Httex1 proteins using novel kinases and SUMO-fusion purification, enabling further study of phosphorylation's role in Httex1 function.

Phosphorylation of the overlooked tyrosine 310 regulates the structure, aggregation, and microtubule- and lipid-binding properties of Tau

This study shows that phosphorylation of tyrosine 310 in Tau reduces its aggregation and microtubule/lipid-binding properties by decreasing β-sheet propensity in the PHF6 domain.

Extent of N-terminus exposure by altered long-range interactions of monomeric alpha-synuclein determines its aggregation propensity

This study investigates how calcium-induced conformational changes in monomeric alpha-synuclein affect N-terminus exposure and aggregation propensity, identifying sequence-specific conformational differences as key factors.