This paper introduces a genetically-encoded c-di-GMP biosensor, cdGreen2, capable of tracking c-di-GMP dynamics in live cells with ultra-high temporal resolution.

bioRxiv · Oct 18, 2022 · PreprintMicrobiology

Extracellular appendages govern spatial dynamics and growth of Caulobacter crescentus on a prevalent biopolymer

V. R. Povolo, G. G. D'Souza, A. Kaczmarczyk, A. K. M. Stubbusch, U. Jenal, M. Ackermann

This study shows that extracellular appendages in Caulobacter crescentus influence spatial dynamics and growth on xylan by affecting cell density and competition.

bioRxiv · Jun 13, 2022 · PreprintMicrobiology

Digital control of c-di-GMP in E. coli balances population-wide developmental transitions and phage sensitivity

A. Reinders, B. Sellner, F. Fadel, M. van Berkum, A. Kaczmarczyk, S. Ozaki, J. Rueher, P. Manfredi, M. Sangermani, A. Harms, C. Perez, T. Schirmer, U. Jenal

This study shows E. coli uses a digital switch to convert c-di-GMP dynamics into binary states, enabling population-level developmental transitions and phage resistance.

bioRxiv · Oct 1, 2021 · PreprintMicrobiology

Single-molecule analysis reveals cooperative stimulation of Rad51 filament nucleation and growth by mediator proteins

O. Belan, C. Barroso, A. Kaczmarczyk, R. Anand, S. Federico, N. O'Reilly, M. D. Newton, E. Maeots, R. I. Enchev, E. Martinez-Perez, D. S. Rueda, S. J. Boulton

The paper uses single-molecule imaging to show how BRC-2 and RFS-1/RIP-1 mediate RAD-51 filament nucleation and growth during homologous recombination.

bioRxiv · Nov 5, 2020Biophysics

Regulation of bacterial cell cycle progression by redundant phosphatases

J. Coppine, A. Kaczmarczyk, K. Petit, T. Brochier, U. Jenal, R. Hallez

The paper identifies CckN as a second phosphatase in Caulobacter crescentus that redundantly regulates CtrA activity with PleC during the G1 phase of the cell cycle.

Journal of Bacteriology · Aug 10, 2020Peer-reviewedMicrobiology

Hybrid histidine kinase activation by cyclic di-GMP- mediated domain liberation

B. N. Dubey, E. Agustoni, R. Boehm, A. Kaczmarczyk, F. Mangia, C. von Arx, U. Jenal, S. Hiller, I. Plaza-Menacho, T. Schirmer

This study reveals how c-di-GMP regulates ShkA, a hybrid histidine kinase in Caulobacter crescentus, by inducing domain dissociation and allosteric activation.

bioRxiv · Jun 22, 2019Biochemistry

Precise transcription timing by a second-messenger drives a bacterial G1/S cell cycle transition

A. Kaczmarczyk, A. M. Hempel, C. von Arx, R. Boehm, B. N. Dubey, J. Nesper, T. Schirmer, S. Hiller, U. Jenal

This study identifies c-di-GMP as a second messenger that regulates the G1/S transition in Caulobacter crescentus by modulating ShkA and TacA activity.

bioRxiv · Jun 20, 2019Microbiology

A single-domain response regulator functions as integrating hub to coordinate general stress response and development in alpha-proteobacteria

C. Lori, A. Kaczmarczyk, I. de Jong, U. Jenal

This paper identifies MrrA as a central phosphorylation hub in Caulobacter crescentus that coordinates general stress response with development by acting as a phosphotransfer relay between multiple histidine kinases and response regulators.

bioRxiv · Apr 11, 2018Microbiology