Cell BiologybioRxiv
Live cell biosensors based on the fluorescence lifetime of environment-sensing dyes
B. Mehl, L. Li, E. Hinde…·K. M. Hahn
This study develops a FLIM biosensor using merocyanine dyes to detect conformational changes in Cdc42, showing improved sensitivity at low activation levels compared to FRET-based methods.
DOI 10.1016/j.crmeth.2024.100734