D. J. Nieves

The paper evaluates SMLM cluster analysis algorithms using ARI and IoU metrics across simulated scenarios to establish a standard framework for future algorithm development.

The paper maps biophysical environments in mammalian cell membranes using solvatochromic probes and single-molecule imaging with nanoscale resolution.

The paper introduces tagPAINT, a covalent labeling method for DNA-PAINT imaging using genetically encoded protein tags, and applies it to study T-cell receptor signaling proteins.

This paper demonstrates that qPAINT measurements remain robust even when target molecules are as close as 3 nm apart, showing no significant cross-reactivity in dense assemblies.

This study shows that Lck's conformational states determine its diffusive behavior in T cells, with open conformations leading to confined diffusion and closed conformations allowing free diffusion.