D. Pincus

Feedback control of the heat shock response by spatiotemporal regulation of Hsp70

The paper disrupts each gene in the yeast heat shock response to identify feedback loops regulating Hsp70 expression and localization, revealing spatiotemporal control mechanisms.

Adaptive preservation of orphan ribosomal proteins in chaperone-stirred condensates

During stress, molecular chaperones preserve 'orphan' ribosomal proteins in liquid-like condensates, maintaining them in a usable form and enabling efficient recovery from stress.

Induction of Sis1 promotes fitness but not feedback in the heat shock response

The paper defines newly synthesized proteins as HSR activators and shows Sis1 induction enhances fitness during stress without providing negative feedback to the HSR.

Subcellular localization of the J-protein Sis1 regulates the heat shock response

The paper shows that the J-protein Sis1's subcellular localization regulates the heat shock response in yeast by modulating Hsp70's interaction with Hsf1.

A risk-reward tradeoff of high ribosome production in proliferating cells

The paper shows that disruptions in ribosome biogenesis in yeast cause proteostasis loss, and enhancing proteostasis improves cell fitness under ribosome assembly stress.

Transient Hsp90 suppression promotes a heritable change in protein translation

The paper shows that transient Hsp90 suppression causes a heritable change in protein translation that persists across generations.

Chaperone AMPylation modulates aggregation and toxicity of neurodegenerative disease-associated polypeptides

This study shows that AMPylation of HSP70 modulates protein aggregation and toxicity in neurodegenerative disease models using C. elegans.

Hsf1 and Hsp70 constitute a two-component feedback loop that regulates the yeast heat shock response

The paper validates a mathematical model of the yeast heat shock response by showing Hsp70-mediated negative feedback regulates Hsf1 activity through specific binding sites and domains.