G. McConnell

A Novel 3D Imaging Approach for Quantification of GLUT4 Levels across the Intact Myocardium

The paper introduces a 3D optical mesoscopy method to quantify GLUT4 levels in intact mouse hearts and shows gender-dependent expression changes with high fat diet.

Fractal complexity of Escherichia coli nutrient transport channels is influenced by cell shape and growth environment

This study quantifies how E. coli biofilm channel complexity changes with cell shape and growth conditions using fractal analysis.

Low-cost 3D printed lenses for brightfield and fluorescence microscopy

The paper presents low-cost 3D printed lenses for brightfield and fluorescence microscopy, demonstrating sub-cellular resolution comparable to commercial optics.

Printing, Characterising, and Assessing Transparent 3D Printed Lenses for Optical Imaging

This study develops a low-cost method to produce high-quality 3D printed lenses comparable to commercial glass lenses for optical imaging applications.

Time-lapse mesoscopy of Candida albicans and Staphylococcus aureus dual-species biofilms reveals a structural role for the hyphae of C. albicans in biofilm formation

The paper uses time-lapse mesoscopy to study dual-species biofilms of Candida albicans and Staphylococcus aureus, showing that C. albicans morphology affects biofilm structure and infection synergy.

Multimodal optical mesoscopy reveals the quantity and spatial distribution of gram-positive biofilms in ex vivo tonsils

The paper uses multimodal optical mesoscopy to quantify and map gram-positive biofilms in pediatric tonsils, finding no significant difference in biofilm presence between surface and interior.

Construction and Characterisation of a Structured, Tuneable, and Transparent 3D Culture Platform for Soil Bacteria

The paper develops a 3D culture platform for soil bacteria that is tunable, transparent, and compatible with imaging, demonstrating its use with Bacillus subtilis and Streptomyces coelicolor.

The murine meninges acquire lymphoid tissue properties and harbour autoreactive B cells during chronic Trypanosoma brucei infection

This study shows that chronic Trypanosoma brucei infection in mice causes meningeal lymphoid aggregates with autoreactive B cells and autoantibodies against brain antigens.

Standing wave mesoscopy

This paper introduces a mesoscale version of standing wave microscopy, enabling high-resolution imaging of large cell populations in fixed and living specimens under flow conditions.

A simple image processing pipeline to sharpen topology maps in multi-wavelength interference microscopy

This paper introduces an image processing pipeline to sharpen topographical maps in multi-wavelength interference microscopy, improving clarity and precision of cellular structures.

MesoTIRF: a Total Internal Reflection Fluorescence illuminator for axial super-resolution membrane imaging at the mesoscale

The paper introduces MesoTIRF, a TIRF illuminator that expands the field of view while maintaining high resolution for membrane imaging.

Application of light-sheet mesoscopy to image host-pathogen interactions in intact organs

This paper uses light-sheet mesoscopy to image neuroinflammation in chronically infected mouse brains, revealing increased GFAP+ cell dendrite branching.

Light-sheet mesoscopy with the Mesolens provides fast sub-cellular resolution imaging throughout large tissue volumes.

The paper develops two light-sheet illuminators for the Mesolens to enable fast, high-resolution imaging of large tissue volumes.

Intra-colony channel morphology in Escherichia coli biofilms is governed by nutrient availability and substrate stiffness

This study shows that nutrient availability and substrate stiffness influence intra-colony channel morphology in E. coli biofilms, with glucose-limited biofilms having wider central channels.

Enhanced fluorescence from semiconductor quantum dot-labelled cells excited at 280 nm

This study compares 280 nm and 365 nm excitation of semiconductor quantum dots in cells, finding higher fluorescence intensity and no increased photobleaching at 280 nm.

An evaluation of multi-excitation-wavelength standing-wave fluorescence microscopy (TartanSW) to improve sampling density in studies of the cell membrane and cytoskeleton

This study evaluates TartanSW, a multi-excitation-wavelength standing-wave fluorescence microscopy method, showing it increases sampling density from 50% to 98% without compromising resolution.

Functional channels in mature E. coli colonies

The paper studies E. coli biofilms using Mesolens to identify functional channels that transport particles and aid nutrient absorption, suggesting new strategies for drug delivery.

Three-dimensional observations of an aperiodic oscillatory gliding motility behaviour in Myxococcus xanthus using confocal interference reflection microscopy

This paper uses confocal interference reflection microscopy to observe 3D aperiodic oscillatory gliding motility in Myxococcus xanthus, revealing previously unseen height changes during movement.

Fast Optical Sectioning for Widefield Fluorescence Mesoscopy with the Mesolens based on HiLo Microscopy

The paper introduces a fast optical sectioning method for widefield fluorescence mesoscopy using HiLo microscopy, achieving subcellular resolution and speeds up to 30 times faster than confocal laser scanning microscopy.