This study evaluates citizen scientists' ability to count Galapagos marine iguanas from drone images, finding high accuracy with proper aggregation methods and image quality filtering.

bioRxiv · Feb 9, 2024 · PreprintZoology

The murine meninges acquire lymphoid tissue properties and harbour autoreactive B cells during chronic Trypanosoma brucei infection

J. F. Quintana Alcala, M. C. Sinton, P. Chandrasegaran, L. K. Dubey, J. Ogunsola, M. A. Samman, M. Haley, G. McConnell, N. R. Kuispond Swar, D. M. Ngoyi, D. Bending, L. de Lecea, A. MacLeod, N. A. Mabbott

This study shows that chronic Trypanosoma brucei infection in mice causes meningeal lymphoid aggregates with autoreactive B cells and autoantibodies against brain antigens.

bioRxiv · Apr 30, 2023 · PreprintImmunology

An Aging-Susceptible Circadian Rhythm Controls Cutaneous Antiviral Immunity

S. Kirchner, V. Lei, P. Kim, M. Patel, J. Shannon, D. Corcoran, D. Hughes, D. Waters, K. Dzirasa, D. Erdmann, J. Coers, A. MacLeod, J. Zhang

This study identifies a circadian rhythm that regulates antiviral immunity in skin, showing that aging disrupts this rhythm and that circadian enhancers can restore antiviral defenses.

bioRxiv · Apr 14, 2023 · PreprintImmunology

Spatially-resolved single cell transcriptomics reveal a critical role for γδ T cells in the control of skin inflammation and subcutaneous adipose wasting during chronic Trypanosoma brucei infection

J. F. Q. Alcala, M. C. Sinton, P. Chandrasegaran, A. N. Lestari, R. Heslop, B. Cheaib, J. Ogunsola, D. M. Ngoyi, N. R. K. Swar, A. Cooper, S. B. Coffelt, A. MacLeod

This study uses spatial and single-cell transcriptomics to show that γδ T cells and adipocytes collaborate to control skin inflammation and adipose tissue wasting during chronic Trypanosoma brucei infection.

bioRxiv · Mar 1, 2023 · PreprintImmunology

FLAgellum Member 8 modulates extravascular distribution of African trypanosomes.

E. Calvo Alvarez, J. M. T. Ngoune, P. Sharma, A. Cooper, A. Camara, C. Travaille, A. Crouzols, A. MacLeod, B. Rotureau

This paper identifies FLAM8 as a flagellar protein that modulates extravascular dissemination of Trypanosoma brucei in mammalian hosts, without affecting transmissible stage differentiation.

bioRxiv · Feb 20, 2023Microbiology

IL-17 signalling is critical for controlling subcutaneous adipose tissue dynamics and parasite burden during chronic Trypanosoma brucei infection

M. C. Sinton, P. Chandrasegaran, P. Capewell, A. Cooper, A. Girard, J. Ogunsola, G. Perona-Wright, D. Ngoyi, N. Kuispond, B. BUCHETON, M. Camara, S. Kajimura, C. Benezech, A. MacLeod, J. F. Quintana Alcala

bioRxiv · Sep 23, 2022Immunology

Integrative single cell and spatial transcriptomic analysis reveal reciprocal microglia - plasma cell crosstalk in the mouse brain during chronic Trypanosoma brucei infection

J. F. Quintana Alcala, P. Chandrasegaran, M. Sinton, E. M. Briggs, T. D. Otto, R. Heslop, C. Bentley-Abbot, C. Loney, L. de Lecea, N. A. Mabbott, A. MacLeod

This study uses single cell and spatial transcriptomics to identify microglia-plasma cell interactions in the mouse brain during chronic T. brucei infection.

bioRxiv · Mar 25, 2022 · PreprintPathology