J. Berro

CRISPR-Cas9 editing efficiency in fission yeast is not limited by homology search and is improved by combining gap-repair with fluoride selection

A mechanical circuit in End4p coordinates force transmission during clathrin-mediated endocytosis

This study identifies a mechanical circuit in End4p that regulates force transmission during clathrin-mediated endocytosis in fission yeast.

Fast actin disassembly and fimbrin mechanosensitivity support rapid turnover during clathrin-mediated endocytosis

The paper develops a stochastic model to explain dwell time distributions of actin and fimbrin during clathrin-mediated endocytosis in yeast, showing fast actin disassembly and mechanosensitive fimbrin detachment.

A model of actin-driven endocytosis explains differences of endocytic motility in budding and fission yeast

The paper uses a 3D model to show that differences in turgor pressure between budding and fission yeast explain their distinct endocytic motility characteristics.

Force redistribution in clathrin-mediated endocytosis revealed by coiled-coil force sensors

The paper measures forces during clathrin-mediated endocytosis in fission yeast using new in vivo force sensors, revealing force redistribution across the endocytic machinery.

Rapid adaptation of endocytosis, exocytosis and eisosomes after an acute increase in membrane tension in yeast cells

This study shows yeast cells rapidly adapt to increased membrane tension through eisosome disassembly, temporary endocytosis reduction, and exocytosis increase.

Endocytosis against high turgor pressure is made easier by partial protein coating and a freely rotating base

This study investigates how high turgor pressure in yeast cells affects clathrin-mediated endocytosis and shows that partial protein coating and a freely rotating base reduce the force required for membrane deformation.

DNA-Origami-Based Fluorescence Brightness Standards for Convenient and Fast Protein Counting in Live Cells

The paper introduces DNA-origami-based fluorescence brightness standards for fast and convenient protein counting in live cells, compatible with various imaging applications.

Single-molecule turnover dynamics of actin and membrane coat proteins in clathrin-mediated endocytosis

The paper measures single-molecule turnover dynamics of actin and membrane coat proteins in clathrin-mediated endocytosis in yeast, revealing rapid turnover and heterogeneous behaviors.

Crosslinking actin networks produces compressive force

The paper shows that crosslinking non-polymerizing actin filaments generates compressive forces in yeast, up to thousands of piconewtons, through Brownian dynamics simulations.

Simulation of the mechanics of actin assembly during endocytosis in yeast

The paper simulates actin assembly mechanics during yeast endocytosis, showing that forces from polymerized actin can overcome turgor pressure.

Conditions for optimal mechanical power generation by a bundle of growing actin filaments

This study develops a biophysical model of actin filament bundles to determine optimal conditions for mechanical power generation under external forces.

Structural organization and energy storage in crosslinked actin-assemblies

This study uses mathematical modeling to show that actin filaments and crosslinkers can form ordered or disordered structures during endocytosis, storing elastic energy that may be used later in the process.

New single-molecule imaging of the eisosome BAR domain protein Pil1p reveals filament-like dynamics

The paper develops a new imaging technique to study Pil1p dynamics in fission yeast, revealing heterogeneous exchange and a filament-like eisosome structure.