D. Baddeley

The nanoscale organization of reticulon 4 shapes local endoplasmic reticulum structure in situ

The paper shows Rtn4 oligomers increase ER membrane curvature by localizing to elliptical tubules and enhancing local density.

Extracting nanoscale membrane morphology fromsingle-molecule localizations

The paper introduces NanoWrap, a method for reconstructing subcellular membrane surfaces from single-molecule localization microscopy data, validated on simulated and experimental data.

An Integrated Platform for High-Throughput Nanoscopy

This paper presents an integrated platform for high-throughput single-molecule localization microscopy (SMLM) that enables imaging of 10,000 cells per day.

PYMEVisualize: an open-source tool for exploring 3D super-resolution data

PYMEVisualize is an open-source tool for interactive exploration and analysis of 3D, multicolor single-molecule localization data from super-resolution microscopy.

Fluorogenic probe for fast 3D whole-cell DNA-PAINT

This paper introduces a fluorogenic DNA-PAINT probe that reduces background and increases imaging speed for 3D whole-cell DNA-PAINT.

3D super-resolution microscopy performance and quantitative analysis assessment using DNA-PAINT and DNA origami test samples

Single-molecule turnover dynamics of actin and membrane coat proteins in clathrin-mediated endocytosis

The paper measures single-molecule turnover dynamics of actin and membrane coat proteins in clathrin-mediated endocytosis in yeast, revealing rapid turnover and heterogeneous behaviors.

Nanoscale subcellular architecture revealed by multicolor 3D salvaged fluorescence imaging

This paper introduces a super-resolution microscope that images whole mammalian cells at ~20 nm 3D resolution, revealing complex cellular structures previously only visible via electron microscopy.

A stable, high refractive index, switching buffer for super-resolution imaging

The paper develops a stable, high refractive index buffer for dSTORM super-resolution imaging, improving stability, switching speed, contrast, and refractive index matching.

Simultaneously measuring image features and resolution in live-cell STED images

The paper introduces NEP fitting to measure resolution and cellular features in live-cell STED images without beads or pre-calibration, validated on microtubules and applied to ER tubules.

New single-molecule imaging of the eisosome BAR domain protein Pil1p reveals filament-like dynamics

The paper develops a new imaging technique to study Pil1p dynamics in fission yeast, revealing heterogeneous exchange and a filament-like eisosome structure.