J. Sufi

SIGNAL-seq: Multimodal Single-cell Inter- and Intra-cellular Signalling Analysis

SIGNAL-seq measures RNA and PTMs in single cells to analyze inter- and intra-cellular signaling in the tumor microenvironment.

A Single-cell Perturbation Landscape of Colonic Stem Cell Polarisation

The paper maps how oncogenic mutations and microenvironmental signals co-regulate colonic stem cell fate through single-cell analysis of organoid cultures.

Trellis Single-Cell Screening Reveals Stromal Regulation of Patient-Derived Organoid Drug Responses

The paper develops Trellis, a method to analyze single-cell drug responses in PDOs and CAFs, revealing patient-specific apoptosis and CAF-mediated chemoprotection via YAP signaling.

Enhanced functionality of low-affinity CD19 CAR T-cells is associated with activation priming and a polyfunctional cytokine phenotype

The paper compares low-affinity and high-affinity CD19 CAR T-cells, finding that low-affinity CARs show enhanced activation and cytokine polyfunctionality due to antigen-dependent priming.

Single-Cell Signalling Analysis of Heterocellular Organoids

This study uses mass cytometry to analyze cell-type specific signaling networks in organoids and reveals how genetic mutations mimic stromal cell signaling in cancer organoids.