K. Ikegami

Excessive F-Actin and Microtubule Formation Mediates Primary Cilia Shortening and Loss in Response to Increased Extracellular Osmotic Pressure

This study shows that hyperosmotic shock causes reversible primary cilia shortening and loss in mouse cells, mediated by excessive microtubule and F-actin formation.

Circadian oscillation in primary cilium length by clock genes regulate fibroblast cell migration

This study shows that circadian rhythms regulate primary cilia length in fibroblasts, which in turn affects cell migration and wound healing.

A mouse model of insomnia with sleep apnea

This paper develops a mouse model of comorbid insomnia and sleep apnea (COMISA) by inducing daytime hypoxia, showing a down-and-up activity response linked to sleepiness and insomnia.

A pair of primers facing at the double-strand break site enables to detect NHEJ-mediated indel mutations at a 1-bp resolution

The paper introduces DST-PCR with TBE-High-Resolution PAGE to detect NHEJ-mediated indel mutations at 1-bp resolution in CRISPR/Cas9-engineered cell lines.

Knock-in of labeled proteins into 5'UTR enables highly efficient generation of stable cell lines

This study develops a CRISPR/Cas9-based method to knock-in tagged proteins into the 5'UTR of endogenous genes, achieving high-efficiency stable cell lines with proper protein localization and long-term expression.

Structural instability and divergence from conserved residues underlie intracellular retention of mammalian odorant receptors

This study identifies structural instability and divergence from conserved residues as causes of intracellular retention of odorant receptors, using computational and experimental methods.

Extensive recombination suppression and chromosome-wide differentiation of a segregation distorter in Drosophila

The paper analyzes the evolutionary dynamics of a segregation distorter in Drosophila pseudoobscura, finding that it persists due to suppressed recombination and epistatic selection.