Author

M. Barcena

Listed by name: these papers carry no ORCID for this author, so different people with the same name may be mixed together.

Biology Disciplines

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Microbiology· 5 Cell Biology· 1

Signature Assays, Organisms & Methods

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Approach:experimental assay· 5 Research Focus:host-pathogen & immune response· 4 Assay:electronic health records· 2 Assay:cryo-EM· 1 Approach:deep learning· 1 Assay:imaging· 1 Research Focus:imputation· 1 Research Focus:software / tool· 1

Frequent Co-Authors

E. J. Snijder· 5 shared A. J. Koster· 4 shared R. W. A. L. Limpens· 4 shared A. W. M. de Jong· 3 shared G. Wolff· 3 shared J. C. Zevenhoven-Dobbe· 3 shared S. Zheng· 3 shared A. H. de Wilde· 2 shared

Activity by Year

2019-2022 · peak 3/yr

Co-Author Collaboration Network

10 top collaborators · 40 mutual co-author links

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5 shared papersMicrobiology

E. J. Snijder

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Author profile →

6 papers

Recent·Most cited

AreTomo: An integrated software package for automated marker-free, motion-corrected cryo-electron tomographic alignment and reconstruction

S. Zheng, G. Wolff, G. Greenan, Z. Chen, F. G. A. Faas, M. Barcena, A. J. Koster, Y. Cheng, D. Agard

AreTomo automates motion-corrected cryo-electron tomographic alignment and reconstruction, enabling high-resolution subtomogram averaging and real-time feedback during data collection.

bioRxiv · Feb 15, 2022Cell Biology

A molecular pore spans the double membrane of the coronavirus replication organelle

G. Wolff, R. W. A. L. Limpens, J. C. Zevenhoven-Dobbe, U. Laugks, S. Zheng, A. W. M. de Jong, R. I. Koning, D. A. Agard, K. Grünewald, A. J. Koster, E. J. Snijder, M. Barcena

The paper identifies a molecular pore in coronavirus replication organelles that facilitates RNA export to the cytosol, suggesting it as a potential drug target.

bioRxiv · Jun 25, 2020Microbiology

SARS-coronavirus-2 replication in Vero E6 cells: replication kinetics, rapid adaptation and cytopathology

N. Ogando, T. Dalebout, J. C. Zevenhoven-Dobbe, R. W. A. L. Limpens, Y. van der Meer, L. Caly, J. Druce, J. de Vries, M. Kikkert, M. Barcena, I. Sidorov, E. J. Snijder

This study compares SARS-CoV-2 and SARS-CoV replication in Vero E6 cells, finding higher viral RNA levels in SARS-CoV-2 but lower infectious progeny, and adaptive mutations in its spike protein.

bioRxiv · Apr 20, 2020Microbiology

A unifying structural and functional model of the coronavirus replication organelle: tracking down RNA synthesis

E. J. Snijder, R. W. A. L. Limpens, A. H. de Wilde, A. W. M. de Jong, J. C. Zevenhoven-Dobbe, H. J. Maier, F. G. A. Faas, A. J. Koster, M. Barcena

This study identifies double-membrane vesicles (DMVs) as the central site for viral RNA synthesis in coronaviruses, providing a unifying model of the replication organelle.

bioRxiv · Mar 24, 2020Microbiology

Mind the gap: micro-expansion joints drastically decrease the bending of FIB-milled cryo-lamellae

G. Wolff, R. W. A. L. Limpens, S. Zheng, E. J. Snijder, D. Agard, A. J. Koster, M. Barcena

This paper introduces micro-expansion joints to reduce lamella bending during cryo-FIB-milling, improving structural studies of biological samples.

bioRxiv · May 31, 2019Microbiology

Inhibition of arterivirus RNA synthesis by cyclophilin inhibitors is counteracted by mutations in replicase transmembrane subunits

A. H. De Wilde, L. Boomaars-van der Zanden, A. W. M. de Jong, M. Barcena, E. J. Snijder, C. C. Posthuma

This study identifies adaptive mutations in nsp2 and nsp5 of equine arteritis virus that confer resistance to cyclophilin inhibitors, revealing their role in the mechanism of action of these antiviral compounds.

bioRxiv · Mar 24, 2019Microbiology