N. Ando

Conformational Landscapes of a Class I Ribonucleotide Reductase Complex during Turnover Reveal Intrinsic Dynamics and Asymmetry

This study uses cryo-EM and deep learning to analyze conformational changes in ribonucleotide reductase during turnover, revealing dynamic asymmetry and transient states essential for catalysis.

Scaling and merging macromolecular diffuse scattering with mdx2

The paper introduces mdx2, a Python toolkit for processing diffuse scattering data from macromolecular crystals, demonstrating multi-crystal scaling and merging workflows with a cubic insulin dataset.

Miffi: Improving the accuracy of CNN-based cryo-EM micrograph filtering with fine-tuning and Fourier space information

The paper improves CNN-based cryo-EM micrograph filtering by using fine-tuning and Fourier space information, resulting in higher prediction accuracy.

Geomagnetic orientation in subterranean mole crickets during burrowing

This study shows mole crickets use the geomagnetic field to orient burrowing directions, aligning with north-south under normal fields and shifting to east-west when fields are rotated.

Processing macromolecular diffuse scattering data

This paper reviews advances in reciprocal space mapping for diffuse scattering analysis and provides a tutorial on using Python tools for data processing.

Development of in-line anoxic small-angle X-ray scattering and structural characterization of an oxygen-sensing transcriptional regulator

This study develops an in-line anoxic small-angle X-ray scattering system to characterize oxygen-induced structural changes in the FNR transcription factor under anoxic conditions.

Conformational switching and flexibility in cobalamin-dependent methionine synthase studied by small-angle X-ray scattering and cryo-electron microscopy

This study uses SAXS and cryo-EM to describe the resting-state conformation of MetH and propose a model for its functional switching.

Robust total X-ray scattering workflow to study correlated motion of proteins in crystals

This paper introduces a workflow for analyzing protein motion in crystals using total scattering, with methods to subtract lattice disorder and validate results.

Inline SAXS-coupled chromatography under extreme hydrostatic pressure

This study demonstrates that reproducible SEC-SAXS can be performed under extreme hydrostatic pressure up to 100 MPa, revealing pressure-induced changes in biomolecular structure and oligomeric state.

Repurposed Drugs Block Toxin-Driven Platelet Clearance by the Hepatic Ashwell-Morell Receptor to Clear Staphylococcus aureus Bacteremia

REGALS: a general method to deconvolve X-ray scattering data from evolving mixtures

REGALS deconvolves X-ray scattering data from evolving mixtures by incorporating prior knowledge and regularization, enabling analysis of complex datasets with unknown components.

The flexible N-terminus of BchL protects its cluster in oxygenic environments and autoinhibits activity

This paper identifies a mechanism in BchL that protects its [4Fe-4S] cluster and autoinhibits activity, with ATP binding relieving inhibition and enabling complex formation with BchNB.

Diffuse X-ray Scattering from Correlated Motions in a Protein Crystal

This study analyzes diffuse X-ray scattering from lysozyme crystals to understand collective atomic motions and their correlations, revealing the need for internal protein dynamics beyond lattice effects.

Convergent Allostery in Ribonucleotide Reductase

This study identifies a new allostery mechanism in Bacillus subtilis RNR through structural analysis, revealing convergent regulation of enzyme activity.