E. Antony

Cryo-EM captures the coordination of long-range allostery and asymmetric electron transfer by a bi-copper cluster in the nitrogenase-like DPOR complex

The paper uses Cryo-EM to reveal how asymmetry and allostery coordinate long-range electron transfer in the DPOR enzyme through a bi-copper cluster.

Wrapping of single-stranded DNA by Replication Protein A and modulation through phosphorylation

This study uses single-molecule techniques to show that RPA partially wraps ssDNA and that phosphorylation at Ser-384 modulates this wrapping.

Rapid long-distance migration of RPA on single stranded DNA occurs through intersegmental transfer utilizing multivalent interactions

This study investigates RPA's long-distance migration on ssDNA using biophysical methods, finding that intersegmental transfer via multivalent interactions enables rapid diffusion under tension.

Generation of site-specifically labelled fluorescent human XPA to investigate DNA binding dynamics during nucleotide excision repair

This study generates a fluorescently labeled human XPA protein to study DNA binding dynamics during nucleotide excision repair.

Fluorescent human RPA to track assembly dynamics on DNA

This paper describes fluorescently modified human RPA to study its dynamic interactions with ssDNA and other proteins.

Interplay of macromolecular interactions during assembly of human DNA polymerase δ holoenzymes and initiation of DNA synthesis

This study uses FRET assays to show how RPA interactions at P/T junctions facilitate Pol δ holoenzyme assembly and DNA synthesis efficiency.

Human hnRNPA1 reorganizes telomere-bound Replication Protein A

The paper shows hnRNPA1 remodels RPA bound to telomeric ssDNA, forming a ternary complex regulated by TERRA.

Homodecameric Rad52 promotes single-position Rad51 nucleation in homologous recombination

This study reveals that homodecameric Rad52 facilitates single-position Rad51 nucleation in homologous recombination through specific structural and interaction features.

An Aurora B-RPA signaling axis secures chromosome segregation fidelity

This study identifies a mitosis-specific regulation of RPA by Aurora B kinase, showing that phosphorylation of RPA70 at Ser-384 is essential for chromosome segregation fidelity and genomic stability.

Mechanistic Insight into AP-Endonuclease 1 Cleavage of Abasic Sites at Stalled Replication Forks

This study investigates APE1 cleavage of abasic sites at replication forks, revealing its mechanism and interaction with RPA.

Dynamic states of eIF6 and SDS variants modulate interactions with uL14 of the 60S ribosomal subunit.

This study identifies key residues in eIF6 and RPL23 that mediate their interaction and shows how mutations in SDS disrupt this interaction, offering a therapeutic target for cancer and SDS.

Rtt105 configurationally staples RPA and blocks facilitated exchange and interactions with RPA-interacting proteins

This study shows Rtt105 binds to RPA, staples it, and blocks RIP interactions without ssDNA, while stabilizing RPA-ssDNA filaments with ssDNA.

A-type lamins are critical for the recruitment of RPA and RAD51 to stalled replication forks to maintain fork stability

This study shows A-type lamins are essential for recruiting RPA and RAD51 to stalled replication forks, preventing replication fork instability during replication stress.

Hydrogen-deuterium exchange reveals a dynamic DNA binding map of Replication Protein A

This study uses HDX-MS to analyze the dynamic DNA binding properties of RPA domains, revealing that DBD-C is most involved in stabilizing RPA on ssDNA.

Substrate sensing institutes sequential and asymmetric electron transfer in the nitrogenase-like DPOR Complex

The paper investigates electron transfer mechanisms in the DPOR complex, showing sequential and asymmetric electron transfer driven by allosteric communication between subunits.

The flexible N-terminus of BchL protects its cluster in oxygenic environments and autoinhibits activity

This paper identifies a mechanism in BchL that protects its [4Fe-4S] cluster and autoinhibits activity, with ATP binding relieving inhibition and enabling complex formation with BchNB.

Elevated Expression of a Functional Suf Pathway in the E. coli BL21(DE3) Cell Line Enhances Recombinant Production of an Iron-Sulfur Cluster Containing Protein.

The paper shows that restoring the Suf pathway in E. coli BL21(DE3) increases production of [Fe-S] cluster-containing proteins.

Dynamics and Selective Remodeling of the DNA Binding Domains of RPA

The paper visualizes RPA's DNA binding domain dynamics and shows how Rad52 modulates DBD-D to enable ssDNA remodeling during homologous recombination.