N. J. Harris

This study identifies the helical domain of p110γ as an allosteric regulator of PI3Kγ activity, showing distinct roles in p110γ-p84 and p110γ-p101 complexes through phosphorylation or nanobody binding.

This study identifies unique regulatory mechanisms by which oncogenic PIK3CA mutations enhance PI3K membrane recruitment and activation through structural reorientations.

This study investigates the GEF activity of TRAPPIII and TRAPPII complexes toward Rab GTPases using biochemical and structural methods, revealing Rab specificity and membrane interaction mechanisms.

This paper uses HDX-MS to characterize nanobodies targeting PI3K{gamma} complexes, identifying epitopes and functional effects on lipid kinase activity and GPCR activation.

This study investigates how mutations in PI3Kγ affect its regulatory dynamics and identifies a strategy for developing selective inhibitors.

The paper uses HDX-MS, mutagenesis, and MD simulations to show that substrate binding and protonation drive conformational changes in a transporter, while inhibitors prevent this transition.