J. P. Bravo

This study characterizes a type III-B CRISPR-Cas system in Haliangium ochraceum, revealing a proteolytic cascade involving SAVED-CHAT and PCaspase that activates immune responses against RNA targets.

This study investigates how SpRY-Cas9 recognizes DNA targets without requiring a PAM sequence, revealing structural and kinetic mechanisms that improve genome editing versatility.

The paper presents a cryo-EM structure of the type I-C Cascade complex and describes how anti-CRISPR proteins inhibit it by blocking DNA binding.

The paper determines the structure of a type III-D CRISPR-Cas effector complex and characterizes its RNA cleavage mechanism, revealing how multi-subunit fusion proteins assemble into an active RNA endonuclease.

The paper reports structures of Cas12a2 to elucidate its activation pathway and mechanism of indiscriminate nuclease activity against various substrates.

This study determines the cryo-EM structures of the DISARM complex DrmAB and elucidates its mechanism of phage DNA recognition and activation.

This study identifies a linear gRNA:DNA duplex conformation that prevents Cas9 activation in the presence of PAM-distal mismatches, offering insights for improving Cas9 specificity.

This study shows remdesivir inhibits SARS-CoV-2 RNA polymerase by blocking RNA translocation after three base incorporations, leading to delayed chain termination.