V. Gradinaru

The paper identifies transcriptional crosstalk between enhancers and promoters delivered by AAVs, showing concatemer formation enables targeted delivery of large genetic cargo for cell-type-specific gene editing.

The paper identifies two new receptors, Ly6c1 and Car4/CA4, for crossing the blood-brain barrier using AAV vectors and computational modeling.

The paper develops AAV vectors with endothelial-specific tropism for brain delivery in rodents and broad tropism in primates, demonstrating their utility in genetic engineering of the blood-brain barrier.

This paper introduces APPRAISE, a method that rapidly ranks engineered proteins by their receptor binding propensity using structure modeling, demonstrating its accuracy on various protein classes.

A prebiotic diet reduces motor deficits and microglial activation in α-synuclein overexpressing mice by altering gut microbiota and microglial states.

This paper introduces AAV.CAP-Mac, an engineered AAV variant that enables efficient systemic brain-wide gene delivery in infant Old World primates.

The paper demonstrates the utility of multi-wavelength analytical ultracentrifugation for analyzing complex biopolymer mixtures and interactions.

The paper develops a scRNA-seq pipeline to characterize AAV tropism and transcriptional responses at single-cell resolution, revealing cell-type-specific targeting and gene expression changes.

This study activates specific gut neurons in mice to show they influence microbiome structure and gut physiology, including bile acids and fecal output, without brain signals.

The paper identifies AAV capsid variants that target the brain with high specificity and avoid liver expression in mice and marmoset non-human primates.

The paper engineers light-gated channelrhodopsins with high photocurrent and light sensitivity, enabling minimally-invasive optogenetics without invasive surgery or implants.