D. Brown

Discovery of active mouse, plant and fungal cytochrome P450s in endogenous proteomes and upon expression in planta.

The paper identifies active cytochrome P450s in mouse, plant, and fungal systems using chemical probes and mass spectrometry, showing their subcellular localization and activity-based labeling.

HybriSeq: Probe-based Device-free Single-cell RNA Profiling

HybriSeq enables sensitive single-cell RNA profiling by combining in situ hybridization, split-pool barcoding, and sequencing to detect transcripts without end bias.

Difference in Differences analysis evaluates the effects of the Badger Control Policy on Bovine Tuberculosis in England

This study uses Difference in Differences analysis to assess the impact of a badger control policy on bovine tuberculosis incidence in England, finding a 56% reduction in TB rates up to four years post-intervention.

Glutathione transferase photoaffinity labeling demonstrates GST activation by safeners and NPR1-independent activation by BTH

The paper introduces a photoaffinity labeling assay to study GST activation in plants, showing safener-induced activation in wheat and BTH-induced activation in Arabidopsis independent of NPR1.

Primate-conserved Carbonic Anhydrase IV and murine-restricted Ly6c1 are new targets for crossing the blood-brain barrier

The paper identifies two new receptors, Ly6c1 and Car4/CA4, for crossing the blood-brain barrier using AAV vectors and computational modeling.

Distinct Quaternary States, Intermediates, and Autoinhibition During Loading of the DnaB-Replicative Helicase by the Phage {lambda}P Helicase Loader

This study identifies two distinct quaternary states of the DnaB-λP loader complex during DNA replication initiation, revealing structural changes and autoinhibition mechanisms.

Ribosomal s6 kinase (RSK) is a mediator of aquaporin 2 S256 phosphorylation and membrane accumulation after EGFR inhibition with erlotinib

This study identifies RSK as the kinase responsible for AQP2 S256 phosphorylation and membrane trafficking induced by erlotinib, distinct from the VP-PKA pathway.

A bi-functional PARP-HDAC inhibitor with activity in Ewing sarcoma

This paper develops a dual PARP and HDAC inhibitor, kt-3283, showing enhanced cytotoxicity and efficacy in Ewing sarcoma models compared to single-agent inhibitors.

ERα-LBD, a novel isoform of estrogen receptor alpha, promotes breast cancer proliferation and endocrine resistance

The paper identifies ER-LBD, a novel estrogen receptor alpha isoform, which promotes breast cancer growth and resistance to endocrine therapy.

Assessing microbial growth of six pure cultures grown anaerobically using optical density, ATP measurements, DNA concentrations and 16S rRNA targeted qPCR to compare commonly used techniques in lab and industry settings

The paper compares four methods for monitoring microbial growth in pure cultures, finding DNA concentration as the most reliable and versatile technique.

Cerebrospinal fluid outflow through skull channels instructs cranial hematopoiesis

The paper shows cerebrospinal fluid exits through skull channels into bone marrow, and bacteria use this route during meningitis to initiate cranial hematopoiesis.

Deep parallel characterization of AAV tropism and AAV-mediated transcriptional changes via single-cell RNA sequencing

The paper develops a scRNA-seq pipeline to characterize AAV tropism and transcriptional responses at single-cell resolution, revealing cell-type-specific targeting and gene expression changes.

The role of migration in mutant evolution in fragmented populations

This study investigates how migration affects mutant distribution in fragmented populations, showing that migration alters probability distributions and can sustain mutant numbers in small demes.

The duration of antibiotic treatment is associated with carriage of toxigenic and atoxigenic strains of Clostridioides difficile in dogs.

This study investigates C. difficile carriage in dogs, finding that antibiotic use increases carriage, including toxigenic strains linked to human disease, but not necessarily causing diarrhoea in dogs.

Versatile labeling and detection ofendogenous proteins using tag-assistedsplit enzyme complementation

This paper introduces TASEC, a method that enhances protein labeling and detection by using split enzyme complementation with orthogonal tags, demonstrating applications in cell enrichment and interaction studies.

HP1 proteins compact DNA into mechanically and positionally stable phase separated domains

This study shows HP1 proteins form phase-separated DNA condensates that are mechanically stable but can be dissolved by HP1β, with differences in DNA compaction linked to their disordered regions.

Improved yellow-green split fluorescent proteins for protein labeling and signal amplification

The paper improves split fluorescent protein systems for better complementation efficiency and signal amplification in mammalian cells.

Enabling out-of-clinic human immunity studies via single-cell profiling of capillary blood

The paper develops a platform for single-cell immune profiling using capillary blood to study gene expression variability across individuals and time.

Active synthesis of type I collagen homotrimer in Dupuytren's fibrosis is unaffected by anti-TNF-α treatment

This study investigates type I collagen synthesis in Dupuytrens disease and finds anti-TNF-α treatment does not reduce collagen production or prevent homotrimer formation.

Selective Vulnerability of Senescent Glioblastoma Cells to Bcl-XL Inhibition

This study identifies Bcl-XL inhibition as a selective vulnerability of senescent glioblastoma cells following radiation or TMZ treatment.

Primary and recurrent glioma patient-derived orthotopic xenografts (PDOX) represent relevant patient avatars for precision medicine

This study shows that glioma PDOX models accurately represent patient tumors and can predict treatment responses in personalized medicine.

Kinase-mediated RAS signaling via membraneless cytoplasmic protein granules

This study identifies membraneless protein granules formed by RTK oncoproteins that activate RAS/MAPK signaling independently of lipid membranes in cancer.

A role for tetracycline selection in the evolution of Clostridium difficile PCR-ribotype 078

The paper investigates the evolutionary history of Clostridium difficile RT078, finding that tetracycline resistance genes, particularly tetM, were acquired independently in multiple clonal expansions, contributing to its increased prevalence.

A role for a conserved kinase in the transcriptional control of methionine biosynthesis in Escherichia coli experiencing sustained nitrogen starvation

This study identifies YeaG as a kinase that regulates methionine biosynthesis in E. coli under sustained nitrogen starvation by modulating the activity of the transcriptional repressor MetJ.

Transcriptomes of major renal collecting-duct cell types in mouse identified by single-cell RNA-Seq

The paper identifies transcriptomes of major renal collecting-duct cell types in mice using single-cell RNA-Seq and creates an online gene-expression database.

PI3K Activation In Neural Stem Cells Drives Tumorigenesis Which Can Be Suppressed By Targeting CREB

This study shows that PI3K pathway activation in neural stem cells initiates tumorigenesis, and targeting CREB reduces tumor growth in a mouse model of high-grade glioma.