Daniel St Johnston

This paper introduces Intrinsic3D, a 3D SMLM method that enables rapid, whole-cell protein mapping with nanometre resolution, demonstrating its application in studying cell adhesion structures.

The paper shows that integrin diversity in Drosophila midgut epithelia enables dynamic adhesion control, with enterocytes competing for basement membrane adhesion and being extruded when less adhesive.

The paper shows that aPKC inhibition in Drosophila follicle cells triggers apical contraction in response to stretching by activating Yurt through a conserved pathway.

The paper investigates the role of E-cadherin adhesion complexes in Drosophila midgut epithelial polarity, finding they limit septate junction extent but are not required for apical-basal polarity.

The paper tracks exocytic vesicle movements in epithelial cells to show microtubule organisation controls apical secretion and reveals spatially organised ECM secretion.

This paper presents an oblique plane microscope compatible with a commercial base, showing minimal impact on resolution and data fidelity despite objective lens displacement.

This paper shows that asymmetric myosin activation polarizes the Drosophila anterior-posterior axis by recruiting and maintaining Par-1 at the posterior cortex.

The paper identifies a molecular mechanism by which Patronin amplifies fusome asymmetry to select the oocyte in the female germline cyst.

This study shows that integrin function is required for maintaining cellular organisation and shape during Drosophila egg chamber morphogenesis, but not for pre-follicle cell polarity.

The paper shows IMP regulates Notch signaling in Drosophila follicle cells by controlling Kuzbanian localization to the apical domain, revealing a new regulatory step in the Notch pathway.