Kento Morimoto

KOnezumi-AID automates gRNA design for Target-AID-mediated gene knockouts, enabling efficient and safe multiplex knockout strategies in mouse and human genomes.

The paper introduces a novel method for functional analysis of non-coding regions by inducing random mutations in target genomic regions using CRISPR and transposase in mouse embryonic stem cells.

This study improves transgenic mouse production efficiency using piggyBac, enabling phenotyping in the founder generation and demonstrating application with bacterial artificial chromosome transgenes.

This paper introduces a universal method for generating knockout mice in multiple inbred strains using zygote electroporation, enabling efficient phenotyping for gene function and disease research.

This paper introduces DAJIN, a machine learning-assisted long-read sequencing method for comprehensive multiplex genotyping of genome editing outcomes, including intended and unintended mutations.

This study identifies EXOC1's role in regulating pseudopod formation in spermatogonia and syncytium formation in spermatocytes in mice, highlighting its interaction with Rac1 and SNAP23/STX2.