Fumihiro Sugiyama

The paper introduces a novel method for functional analysis of non-coding regions by inducing random mutations in target genomic regions using CRISPR and transposase in mouse embryonic stem cells.

This study improves transgenic mouse production efficiency using piggyBac, enabling phenotyping in the founder generation and demonstrating application with bacterial artificial chromosome transgenes.

This study shows that reducing ooplasmic volume in oocytes improves meiotic accuracy and embryo development, enabling offspring production from azoospermic mice with spermatocyte arrest.

This paper introduces DAJIN, a machine learning-assisted long-read sequencing method for comprehensive multiplex genotyping of genome editing outcomes, including intended and unintended mutations.

The paper identifies cell-type specific genes in olfactory bulb projection neurons using single-cell RNA-seq and validates them as Cre-driver markers for genetic labeling.

This study identifies EXOC1's role in regulating pseudopod formation in spermatogonia and syncytium formation in spermatocytes in mice, highlighting its interaction with Rac1 and SNAP23/STX2.

The paper re-evaluates the efficiency of a CRISPR-Cas9 method for generating conditional knockout alleles in mice, finding very low success rates and high rates of undesired alleles.