Brian Raught

This study shows that PLK1 inhibition eradicates quiescent leukemia stem cells through a non-canonical mechanism involving microtubule and endolysosomal disruption, distinct from its canonical mitotic function.

This study identifies S-palmitoylation as a reversible regulatory mechanism for GDH, affecting its quaternary structure and enzymatic activity through cysteine modifications.

The paper identifies Sec23b as a regulator of cell migration by controlling collagen I secretion and processing through a proteomic-based assay.

The paper presents a chemical-genetic interaction matrix derived from CRISPR/Cas9 screens, revealing drug mechanisms and genetic architecture in human cells.

This paper introduces ProtacID, a BioID-based method to identify PROTAC-proximal proteins, validates degradation targets, and identifies non-productive interactions in human cells.

This study uses cryo-EM to show AKAP350 forms fibrillar clusters and interacts with DNA and DNA-binding proteins like NFIB and NCL.

The paper identifies RAD54L2 as a regulator of recombination by showing its interaction with the BTRR complex and its role in suppressing sister chromatid exchanges.

The paper identifies cytosolic mutant proteins degraded by the proteasome and shows DNAJA2 stabilizes some mutants while being dispensable for wild-type proteins.

The paper identifies TBPL1 and PAF1 as regulators of Pol II at the intergenic spacer (IGS) of rDNA, showing their role in controlling Pol I activity and maintaining nucleolar structure and rRNA biogenesis.

This paper describes a targeted protein degradation strategy using UNC8732 to recruit FBXO22 for NSD2 degradation, leading to growth suppression and reversal of drug resistance in leukemia cells.

This study identifies mitochondrial calcium utilization as a metabolic requirement of venetoclax-resistant acute myeloid leukemia stem cells and shows that targeting mitochondrial calcium uptake can eradicate these cells.

This study identifies APC's role in SLIT2-induced ROBO1 endocytosis and its impact on cell morphology.

The paper identifies ACE2 as the only common host dependency for SARS-CoV-2 across multiple cell lines, while other host factors are cell line specific.

The paper maps EMT transitions through multi-omic profiling, identifies key regulatory factors, and reveals discordance between molecular layers in human mammary epithelial cells.

Profiling 192 subcellular markers by proximity-dependent biotinylation (BioID) in human HEK 293 cells maps 4,145 proteins across 32 compartments via 35,902 interactions, available with analysis tools at humancellmap.org.

This study identifies how SLAP2 binding to CBL disrupts its autoinhibition, revealing a mechanism for activating CBL's ubiquitin ligase function.

Proximity-dependent biotinylation of 27 SARS-CoV-2 proteins in A549 cells maps 7810 virus-host proximity interactions across 2242 host proteins, including membrane-associated partners missed by affinity purification.

The paper identifies 2,280 essential genes in human B-cell lymphoma cells using an EKO library and RANKS tool, revealing context-specific essentiality and evolutionary patterns.