Hiroaki Ohishi

The paper shows that chromatin modifications around genes fluctuate with transcriptional activity in mouse embryonic stem cells, revealing minute-scale coupling between histone marks and transcriptional bursts.

The paper uses single-molecule imaging to show RNA fluctuations modulate SOX2 dynamics at the Nanog locus, revealing RNA-dependent feedback in transcriptional regulation.

The paper introduces PECAbs for high-specificity multiplexed imaging of cell states and combines them with seq-smFISH to classify cells and identify signal activation states in human tissue.

The paper examines enhancer-promoter interactions in mouse embryonic stem cells, finding that transcriptional activity increases genomic viscosity, stabilizing these interactions.

This study examines the roles of ADD domains in DNMT3A and DNMT3L in DNA methylation in mouse germ cells, finding that their combined loss leads to severe methylation defects.

The paper develops a STREAMING-tag system to study spatiotemporal relationships between transcriptional factors and activity in living cells.