Pohl Milón

This study identifies optimal buffer conditions for LbCas12a trans-cleavage activity, showing that while some buffers enhance catalytic efficiency, they also increase nonspecific cleavage, requiring careful optimization for detection performance.

The paper develops a portable fluorescence platform for decentralized monitoring of mcr-1, achieving high sensitivity and concordance with sequencing and phenotypic tests.

This paper introduces a fluorescence-based microscale platform to study bacterial translation initiation, revealing the dissociation constant of streptomycin and the effect of antimicrobial peptides.

This study develops C12a, a CRISPR/Cas12a-based tool for detecting antibiotic resistance markers with high sensitivity and concordance across multiple assays.

This study determines the structures of 30S initiation complexes inhibited by kasugamycin, edeine, and GE81112, revealing distinct mechanisms of action within the E-site.

This study uses 4D crystallography to capture transient dynamics of IF1 binding to the 30S ribosomal subunit, revealing allostery and interactions during translation initiation.

This study determines the structure of the 50S ribosomal subunit from Thermus thermophilus at ambient temperature using serial femtosecond X-ray crystallography, revealing new dynamics and antibiotic binding sites.

This paper develops CRISPR-based assays for detecting Leishmania species, showing high sensitivity and specificity for genus and subgenus level identification.

This study develops RNA beacons to detect the testis-determining factor (TDF) using in vitro evolved aptamers and shows their binding kinetics and structural interactions.

This study reveals the kinetic mechanisms and conformational changes of bacterial IF3 during translation initiation.