Author

Tobias Hub

Heidelberg UniversityORCID 0000-0001-9252-8651Unclaimed ORCID profile · follow to get notified of new preprints

Biology Disciplines

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Signature Assays, Organisms & Methods

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Organism:mouse· 2 Research Focus:cell type annotation· 1 Approach:experimental assay· 1 Approach:genetic perturbation / CRISPR· 1

Frequent Co-Authors

Marc FreicheliD· 2 shared Rebekka MedertiD· 2 shared Alex CorneaniD· 1 shared Frank Zimmermann· 1 shared Joachim WittbrodtiD· 1 shared Kellen RoundiD· 1 shared Sascha Dlugosz· 1 shared Tanja Kellner· 1 shared

Activity by Year

2022-2024 · peak 1/yr

Co-Author Collaboration Network

10 top collaborators · 30 mutual co-author links

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2 shared papersCell Biology

Marc FreichelORCID

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2 papers

Recent·Most cited

Cell BiologyACS Pharmacology & Translational SciencePeer-reviewedMay 2, 2024

Streamlined generation of CRISPR/Cas9-mediated single-cell knockout clones in murine cell lines.

Tobias Hub, Alex Cornean, Kellen Round…·Rebekka Medert

This paper describes a method to efficiently generate and identify CRISPR/Cas9-mediated single-cell knockout clones in murine cell lines within 3-4 weeks.

4 citations

mousegenetic perturbation / CRISPR

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Molecular BiologyNucleic Acids ResearchPeer-reviewedDec 12, 2022

Efficient single copy integration via homology-directed repair (scHDR) by 5'modification of large DNA donor fragments in mice

Rebekka Medert, Thomas Thumberger, Tinatini Tavhelidse…·Marc Freichel

This study demonstrates that 5-biotinylation of donor DNA enhances single-copy HDR in mice, achieving 19-26% efficiency in generating conditional alleles.

15 citations

mouseexperimental assay

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