Isaac B. Hilton

This study designs synthetic activators for programmable gene expression by engineering coactivator-binding proteins and demonstrates their enhanced transcriptional activation capabilities.

The paper uses CRISPRa to show that transcriptional memory can be induced in MSCs through locus-specific activation, linked to H3K27me3 loss and increased transcription factor reacquisition.

This study identifies syndecan-3 as a regulator of inflammation and EndMT in aortic valve endothelial cells, showing its role in suppressing NF-kappaB activation and mesenchymal transition.

The paper trains machine learning models to predict gene expression from histone PTMs using ENCODE data and validates predictions with CRISPR-Cas9-based epigenome editing experiments.

This paper develops a method to fluorescently label and track non-repetitive genomic loci in live human cells, showing gene-specific chromatin dynamics independent of transcription.

This paper engineers modified p300 variants fused to dCas9 to reduce cytotoxicity and enhance enzymatic specificity in CRISPR-based epi-editing.

This paper introduces a genetic priming method using IRF1 to sustainably enhance MSC potency and suppress immunogenicity compared to biochemical priming.

The paper analyzes temporal changes in chromatin structure and gene expression during macrophage activation, finding a reciprocal relationship between loops and transcription.

This paper describes the generation of two transgenic mouse lines for in vivo epigenome editing using CRISPR-based systems to activate or repress gene expression.