Gustavo Scanavachi

This study tracks antisense oligonucleotides (ASOs) through endocytic trafficking, finding that productive escape occurs early in endosomes before sequestration in late compartments.

This paper shows that inhibiting PIKfyve causes endosomal swelling, blocking viral entry by preventing fusion and genome release, while sparing VSV-G viruses.

SpatialDINO enables automated segmentation and tracking of crowded cellular objects using self-supervised 3D vision transformers without manual annotations or retraining.

This study shows that tau seeding in neurons occurs via transient endolysosomal perforations that remain confined within endolysosomes and require PIKfyve activity.

This paper introduces DeepSPT, a deep learning framework that automates the analysis of sub-cellular diffusion to infer functional information, achieving high accuracy in identifying endosomal structures during viral infections.

The paper shows that neurons have constitutive endolysosomal perforations enabling internalized alpha-synuclein fibrils to induce aggregation, and inhibiting PIKfyve kinase reduces this effect.

This study confirms that the rotavirus VP5*/VP8* conformational transition enables membrane permeabilization to Ca2+ through C-terminal segment insertion.

This study maps the spatiotemporal dynamics of Notch2 signaling from membrane to nucleus after ligand activation, identifying key molecular events in signal transmission.