Tomas L. Kirchhausen

This study tracks antisense oligonucleotides (ASOs) through endocytic trafficking, finding that productive escape occurs early in endosomes before sequestration in late compartments.

This study uses cryogenic electron tomography to visualize the mechanism of membrane perforation during rhesus rotavirus entry, linking structural data with live-cell imaging.

The paper introduces MOSAIC, a multimodal adaptive optical microscope that enables non-invasive imaging across biological scales in live specimens.

This paper shows that inhibiting PIKfyve causes endosomal swelling, blocking viral entry by preventing fusion and genome release, while sparing VSV-G viruses.

SpatialDINO enables automated segmentation and tracking of crowded cellular objects using self-supervised 3D vision transformers without manual annotations or retraining.

This study shows that tau seeding in neurons occurs via transient endolysosomal perforations that remain confined within endolysosomes and require PIKfyve activity.

The paper uses FIB-SEM and ASEM to identify and characterize vesicular ER exit sites in mammalian cells, revealing their abundance and organization.

This paper introduces DeepSPT, a deep learning framework that automates the analysis of sub-cellular diffusion to infer functional information, achieving high accuracy in identifying endosomal structures during viral infections.

CryoSamba improves cryo-ET image quality by denoising 3D volumes through self-supervised deep learning without requiring labels or synthetic data.

The paper shows that neurons have constitutive endolysosomal perforations enabling internalized alpha-synuclein fibrils to induce aggregation, and inhibiting PIKfyve kinase reduces this effect.

This study tracks Notch and Delta interactions in real-time, showing 1:1 stoichiometry at synapses and NICD nuclear entry after 45 minutes, inhibited by signaling blockers.

This study maps the spatiotemporal dynamics of Notch2 signaling from membrane to nucleus after ligand activation, identifying key molecular events in signal transmission.

This study shows phosphorylation of Liprin-3 triggers phase separation to regulate presynaptic active zone structure and function.

The paper combines expansion and lattice light sheet microscopy to enable high-speed, nanoscale molecular imaging of neural circuits across large volumes.

The paper identifies a pressure relief valve in the endolymphatic sac of zebrafish larvae that regulates inner ear pressure through dynamic lamellar junctions.