Multishot Tomography for High-Resolution In Situ Subtomogram Averaging.

Sagar Khavnekar; William Wan; Parijat Majumder; Wojciech Wietrzyñski; Philipp S. Erdmann; Jürgen M. Plitzko

doi:10.1016/j.jsb.2022.107911

Molecular BiologyJournal of Structural BiologyNov 4, 2022

Peer-reviewed journal article

Multishot Tomography for High-Resolution In Situ Subtomogram Averaging.

Sagar Khavnekar iD, William Wan iD, Parijat Majumder iD, Wojciech Wietrzyñski iD, Philipp S. Erdmann iD, Jürgen M. Plitzko iD

Abstract

Cryo-electron tomography (cryo-ET) and subtomogram averaging (STA) can resolve protein complexes at near atomic resolution, and when combined with focused ion beam (FIB) milling, macromolecules can be observed within their native context. Unlike single particle acquisition (SPA), cryo-ET can be slow, which may reduce overall project throughput. We here propose a fast, multi-position tomographic acquisition scheme based on beam-tilt corrected beam-shift imaging along the tilt axis, which yields sub-nanometer in situ STA averages.

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