Stacy Alyse Malaker

This study performs N-glycoproteomic profiling of Ancylostoma ceylanicum, identifying stage-specific glycoproteins and phosphorylcholine-modified glycans in larval and adult parasites.

This paper identifies the HCA antibody's target as a semenogelin-galectin-3 complex on the sperm surface, rather than CD52, through affinity purification and glycan analysis.

This study uses proteomic analysis in aged rhesus macaques to show that GCPII inhibition alters region-specific protein expression patterns linked to Alzheimer's disease pathology.

This study investigates how site-specific O-glycans on lacritin influence its structure and multimerization, revealing their role in conformational flexibility and crosslinking residue positioning.

This study shows that Pcpe2 in adipose tissue contributes to inflammation and fibrosis in obesity and that its absence reduces these effects and enhances adipocyte differentiation.

This study shows B3GNT7 regulates colonic mucus glycosylation, protects against colitis and infection, and has a distinct function from other B3GNT family members.

This paper maps mucin glycoforms in tumors using on-tissue digestion and mass spectrometry, revealing distinct glycosylation patterns in colorectal mucinous adenocarcinomas.

This study shows that loss of NgBR/cis-PTase function in the liver leads to fatty liver disease that progresses to hepatocellular carcinoma with 100% penetrance, and DGAT2 inhibition prevents this progression.

This paper develops a chemical genetic strategy to manipulate proteoglycan glycan attachment sites in mammalian cells using modified xylosyltransferases.

This study identifies new Gal-3 ligands in tear fluid, showing that lacritin's glycoforms and spliceoforms specifically bind Gal-3, influencing its biological functions.

The paper develops Siglec-liposomes to detect Siglec ligands and modulate immune cell function, showing they enhance T cell proliferation.

This study uses mass spectrometry to analyze tear fluid glycoproteins, revealing novel glycosylation patterns and spliceoforms of lacritin and other clinically relevant proteins.

This study quantifies co-occupancy of N- and O-glycopeptides in mucins and other glycoproteins, showing higher prevalence in mucins and the need for electron-based methods for accurate site-specific analysis.

The paper optimizes a mucin-selective enrichment strategy to improve throughput, reduce sample input, and enhance glycopeptide signal for mucinome analysis.

The paper introduces BOCTAG, a method to label glycoproteins in specific cell types within mixed cultures, enabling analysis of cell-specific glycosylation.

This study compares glycoproteomics search algorithms, finding that O-Pair excels in O-glycopeptide identification while Byonic and pGlyco perform best for N-glycoproteomics.

This paper introduces GalNAzMe, a monosaccharide probe that selectively labels O-GalNAc glycosylation in living cells, enabling detailed study of this cancer-relevant modification.

This study compares rAAV production methods using different host cells, finding chemically and functionally distinct vectors with differences in PTMs, methylation, and potency.

The paper introduces a method to chemically tag glycans in living cells using engineered glycosyltransferases to study glycan structure and biosynthesis.

The paper identifies N-glycan modifications on mammalian Y RNAs, showing RNA can be a scaffold for glycosylation in the secretory pathway.