Structural basis of ZP2-targeted immunocontraception
Elisa DioguardiiD, Alena StsiapanavaiD, Eileen Fahrenkamp, Ling HaniD, Daniele de SanctisiD, José InzunzaiD, Luca JovineiD
Abstract
Monoclonal antibody IE-3 prevents mouse fertilization by binding ZP2, a major component of the oocyte-specific zona pellucida (ZP). We show that an IE3-derived single-chain variable fragment (scFV) is sufficient for blocking fertilization in vitro and determine the structural basis of IE-3/ZP2 recognition. The high-affinity of this interaction depends on induced fit of the epitope, offering insights for non-hormonal immunocontraceptive design without off-target effects.
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