Daniele de Sanctis

This study uses anaerobic time-resolved serial crystallography to capture CO dissociation and rebinding in [FeFe]-hydrogenase, revealing a rigid catalytic architecture.

The paper introduces a 3D-printed fixed-target platform for macromolecular crystallography that simplifies microcrystal handling and data collection at synchrotrons.

This paper introduces a polymer film technology for serial macromolecular crystallography that provides an efficient hydration barrier and low X-ray background.

This study shows that an IE3-derived scFV blocks fertilization by binding ZP2 and determines the structural basis of this interaction, offering insights for non-hormonal immunocontraceptives.

This study presents room-temperature structures of PBP2a using SFX to reveal dynamic features and conformational changes relevant to antibiotic resistance.

This paper combines MicroED and native mass spectrometry to determine structures of enzyme-inhibitor complexes and identify high-affinity ligands from crude samples.

This paper demonstrates how the crystallophore TbXo4 accelerates protein crystallization, enabling high-quality crystals for serial crystallography methods.

This study investigates the metal ion activation and DNA recognition mechanisms of DR2539 in Deinococcus radiodurans using biophysical analyses and structural data.

This study determines the structure of hNQO1 with NADH using serial crystallography and simulations, showing NADH binding stabilizes the protein and drives functional cooperativity through structural communication.

This paper develops a droplet microfluidics approach to produce uniform crystals and enable rapid micromixing for time-resolved serial crystallography.

This study investigates how cleavage of ZP2 in the egg zona pellucida leads to oligomerization and structural changes that block polyspermy.